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  • Primary Antibodies ›
  • MTERF Antibodies

Invitrogen

MTERF Polyclonal Antibody

View all (8) MTERF antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite MTERF Polyclonal Antibody

  • Antibody Testing Data (3)
MTERF Antibody in Immunocytochemistry (ICC/IF)
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MTERF Antibody in Immunocytochemistry (ICC/IF)
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MTERF Antibody (PA5-101935) in ICC/IF

Immunocytochemistry analysis of MTERF in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with MTERF polyclonal antibody (Product # PA5-101935) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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MTERF Antibody in Immunocytochemistry (ICC/IF)
MTERF Antibody in Immunocytochemistry (ICC/IF)
MTERF Antibody in Western Blot (WB)
MTERF Polyclonal Antibody

Product Details

PA5-101935

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human MTERF1(Accession Q99551), corresponding to amino acid residues N266-S316.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2851367

Product Specific Information

Antibody detects endogenous levels of total MTERF.

Target Information

Transcription termination factor. Binds to a 28 bp region within the tRNA(Leu(uur)) gene at a position immediately adjacent to and downstream of the 16S rRNA gene; this region comprises a tridecamer sequence critical for directing accurate termination. Binds DNA along the major grove and promotes DNA bending and partial unwinding. Promotes base flipping. Transcription termination activity appears to be polarized with highest specificity for transcripts initiated on the light strand.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Mitochondrial transcription termination factor 1; mTERF; Transcription termination factor 1, mitochondrial; transcription termination factor, mitochondrial

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Gene Aliases: MTERF; MTERF1

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UniProt ID: (Human) Q99551, (Rat) Q9EPI8

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Entrez Gene ID: (Human) 7978, (Rat) 85261

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Function(s)
double-stranded DNA binding protein binding poly(A) RNA binding
Process(es)
DNA-templated transcription, termination regulation of transcription, DNA-templated termination of mitochondrial transcription DNA geometric change mitochondrion organization
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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