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  • MSK2 Antibodies

Bethyl Laboratories

MSK2 Polyclonal Antibody

View all (11) MSK2 antibodies

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Datasheet
Protocols
Questions & Answers

Cite MSK2 Polyclonal Antibody

  • Antibody Testing Data (2)
MSK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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MSK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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MSK2 Antibody (A302-746A) in IHC (P)

Detection of human MSK2 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MSK2 (Product # A302-746A) used at a dilution of 1:200 (1 µg/mL) Detection: DAB. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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MSK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
MSK2 Antibody in Western Blot (WB)
MSK2 Polyclonal Antibody

Product Details

A302-746A

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000-1:10,000
-

Immunohistochemistry (IHC)

1:100-1:500
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500
-

Immunoprecipitation (IP)

2-5 µg/mg lysate
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 722 and 772 of human Mitogen- and Stress-activated Protein Kinase 2.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.20 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.0 to 8.0, with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

RPS6KA4 (MSK2) is a serine/threonine protein kinase that is essential for the regulation of various cellular processes. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and c-fos. RPS6KA4 contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and c-fos. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 90 kDa ribosomal protein S6 kinase 4; EC 2.7.11.1; kinase MSK2; KS6A4; mitogen- and stress-activated protein kinase 2; mitogen- and stress-activated protein kinase-2; Nuclear mitogen- and stress-activated protein kinase 2; Ribosomal protein kinase B; ribosomal protein S6 kinase alpha 4; Ribosomal protein S6 kinase alpha-4; ribosomal protein S6 kinase, 90kD, polypeptide 4; ribosomal protein S6 kinase, 90kDa, polypeptide 4; RLSK; RSK-like protein kinase; RSKB; S6K-alpha-4

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Gene Aliases: 1110069D02Rik; 90kDa; AI848992; mMSK2; MSK2; RPS6KA4; RSK-B; S6K-alpha-4

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UniProt ID: (Human) O75676, (Mouse) Q9Z2B9

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Entrez Gene ID: (Human) 8986, (Mouse) 56613

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Function(s)
magnesium ion binding protein serine/threonine kinase activity ribosomal protein S6 kinase activity protein binding ATP binding histone kinase activity (H3-S10 specific) mitogen-activated protein kinase p38 binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups
Process(es)
regulation of transcription, DNA-templated protein phosphorylation inflammatory response phosphorylation positive regulation of histone phosphorylation positive regulation of histone acetylation intracellular signal transduction histone H3-S10 phosphorylation histone H3-S28 phosphorylation positive regulation of transcription from RNA polymerase II promoter interleukin-1-mediated signaling pathway negative regulation of cytokine production histone phosphorylation positive regulation of CREB transcription factor activity positive regulation of NF-kappaB transcription factor activity
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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