Invitrogen

MSH6 Recombinant Rabbit Monoclonal Antibody (SP08-02)

Name: MSH6 Recombinant Rabbit Monoclonal Antibody (SP08-02)
Brand: Invitrogen
SKU: MA5-32040

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Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (9)
Advanced Verification (2)

MSH6 Antibody in Immunocytochemistry (ICC/IF)

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MSH6 Antibody (MA5-32040) in ICC/IF

Immunocytochemical analysis of MSH6 in A549 cells using a MSH6 Monoclonal antibody (Product # MA5-32040) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

MSH6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

MSH6 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of MSH6 was observed in all cell lines and tissue lysates in comparison to Jurkat and LNCaP using MSH6 Recombinant Rabbit Monoclonal Antibody (SP08-02) (Product # MA5-32040) in western blot. {RE}

Immunogen image

Immunogen Image MSH6 Recombinant Rabbit Monoclonal Antibody (SP08-02)

Product Details

MA5-32040

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:50-1:100

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SP08-02

Immunogen

Synthetic peptide within Human MSH6 aa 1-50

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2809334

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

The DNA mismatch repair MSH2/MSH6 heterodimer is responsible for the detection of DNA damage, and may promote apoptosis in normal cells, thus protecting mammals from ultraviolet-induced malignant transformation and hence the prevention of skin cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DNA mismatch repair protein Msh6; G/T mismatch-binding protein; GTBP; hMSH6; mutS (E. coli) homolog 6; MutS alpha 160kDa subunit; mutS homolog 6; MutS protein homolog 6; MutS-alpha 160 kDa subunit; MutS-alpha 160kDa subunit; p160; rCG61559-like; Sperm associated protein; sperm-associated protein

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Gene Aliases: AU044881; AW550279; GTBP; GTMBP; HNPCC5; HSAP; MSH6; p160

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UniProt ID: (Human) P52701, (Mouse) P54276

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Entrez Gene ID: (Rat) 100360342, (Human) 2956, (Mouse) 17688

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Function(s)

magnesium ion binding four-way junction DNA binding DNA binding chromatin binding damaged DNA binding double-stranded DNA binding ATP binding ATPase activity mismatched DNA binding guanine/thymine mispair binding single guanine insertion binding single thymine insertion binding oxidized purine DNA binding MutLalpha complex binding methylated histone binding protein homodimerization activity ADP binding protein binding nucleotide binding DNA-dependent ATPase activity single base insertion or deletion binding

Process(es)

meiotic mismatch repair DNA repair mismatch repair cellular response to DNA damage stimulus reciprocal meiotic recombination determination of adult lifespan intrinsic apoptotic signaling pathway in response to DNA damage response to UV somatic hypermutation of immunoglobulin genes somatic recombination of immunoglobulin gene segments maintenance of DNA repeat elements isotype switching negative regulation of DNA recombination positive regulation of helicase activity intrinsic apoptotic signaling pathway viral process positive regulation of isotype switching

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MSH6 Recombinant Rabbit Monoclonal Antibody (SP08-02)

MSH6 Antibody (MA5-32040) in ICC/IF

Product Details

MA5-32040

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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