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  • Primary Antibodies ›
  • MICA/MICB Antibodies

Invitrogen

MICA/B Polyclonal Antibody

View all (17) MICA/MICB antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite MICA/B Polyclonal Antibody

  • Antibody Testing Data (7)
MICA/B Antibody in Immunocytochemistry (ICC/IF)
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MICA/B Antibody in Immunocytochemistry (ICC/IF)
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FIGURE: 1 / 7

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MICA/B Antibody (PA5-109323) in ICC/IF

Immunofluorescent analysis of MICA + MICB in A431 cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with MICA + MICB polyclonal antibody (Product # PA5-109323) at a dilution of 1:100 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:100. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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MICA/B Antibody in Immunocytochemistry (ICC/IF)
MICA/B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
MICA/B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
MICA/B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
MICA/B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
MICA/B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
MICA/B Antibody in Western Blot (WB)
MICA/B Polyclonal Antibody

Product Details

PA5-109323

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:50-1:100
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein within Human MICA + MICB aa 182-325
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 0.2% BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2854734

Product Specific Information

Positive Control: U937, Mouse bone marrow, A431, rat skin tissue, human lung cancer tissue, human colon tissue, human breast carcinoma tissue, mouse stomach tissue.

Target Information

MICA encodes the higly polymorphic MHC (HLA) class I chain-related gene A. The protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: HLA class I antigen; MHC class I chain-related protein A; MHC class I chain-related protein B; MHC class I mic-B antigen; MHC class I polypeptide-related sequence A; MHC class I polypeptide-related sequence B; MHC class I related sequence A; MHC class I-like molecule PERB11.2-IMX; MIC-A; MIC-B; MICA; MICB; sMICA; sMICB; soluble MICA; soluble MICB; stress inducible class I homolog; truncated MHC class I polypeptide-related sequence A

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Gene Aliases: MIC-A; MICA; MICB; PERB11.1; PERB11.2

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UniProt ID: (Human) Q9UE11, (Human) Q29980

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Entrez Gene ID: (Human) 100507436, (Human) 4277

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Function(s)
antigen binding protein binding beta-2-microglobulin binding natural killer cell lectin-like receptor binding
Process(es)
T cell mediated cytotoxicity immune response to tumor cell cellular response to DNA damage stimulus response to heat cytolysis antigen processing and presentation negative regulation of natural killer cell activation natural killer cell mediated cytotoxicity defense response to bacterium negative regulation of natural killer cell mediated cytotoxicity gamma-delta T cell activation regulation of immune response defense response to virus immune response-activating cell surface receptor signaling pathway response to oxidative stress viral process response to retinoic acid negative regulation of defense response to virus by host
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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