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  • MGST1 Antibodies

Invitrogen

MGST1 Polyclonal Antibody

View all (10) MGST1 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite MGST1 Polyclonal Antibody

  • Antibody Testing Data (5)
MGST1 Antibody in Immunocytochemistry (ICC/IF)
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MGST1 Antibody in Immunocytochemistry (ICC/IF)
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FIGURE: 1 / 5

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MGST1 Antibody (PA5-116438) in ICC/IF

Immunocytochemical analysis of MGST1 in hepG2 cells using a MGST1 polyclonal antibody (Product #PA5-116438). Formalin fixed cells, as seen in green, were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody ( 1:100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:100 dilution. The nuclear counter stain is DAPI (blue). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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MGST1 Antibody in Immunocytochemistry (ICC/IF)
MGST1 Antibody in Immunocytochemistry (ICC/IF)
MGST1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
MGST1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
MGST1 Antibody in Western Blot (WB)
MGST1 Polyclonal Antibody

Product Details

PA5-116438

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

Flow Cytometry (Flow)

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein within Human MGST1 aa 1-144
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 0.2% BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2901070

Product Specific Information

Positive controls: Siha cell lysate, HepG2 cell lysate, A549 cell lysate, mouse stomach tissue lysate, HepG2, siha, Rat Adrenal gland tissue, Human liver cancer tissue.

Target Information

The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, two of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. Other family members, demonstrating glutathione S-transferase and peroxidase activities, are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. This gene encodes a protein that catalyzes the conjugation of glutathione to electrophiles and the reduction of lipid hydroperoxides. This protein is localized to the endoplasmic reticulum and outer mitochondrial membrane where it is thought to protect these membranes from oxidative stress.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: glutathione S-transferase 12; MGC14525; Microsomal glutathione S-transferase 1; Microsomal GST-1; Microsomal GST-I

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Gene Aliases: 1500002K10Rik; Gst; GST12; MGST; MGST-I; MGST1

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UniProt ID: (Human) P10620, (Mouse) Q91VS7, (Rat) P08011

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Entrez Gene ID: (Human) 4257, (Mouse) 56615, (Rat) 171341

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Function(s)
glutathione transferase activity glutathione peroxidase activity protein binding protein homodimerization activity glutathione binding transferase activity identical protein binding
Process(es)
glutathione metabolic process response to organonitrogen compound response to lipopolysaccharide response to drug oxidation-reduction process protein homotrimerization cellular response to lipid hydroperoxide xenobiotic metabolic process Leydig cell differentiation cellular oxidant detoxification glutathione derivative biosynthetic process
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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