Invitrogen

MFN2 Polyclonal Antibody

Name: MFN2 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-118059

Advanced Verification

View all (38) MFN2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (6)
Advanced Verification (1)

MFN2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

MFN2 Antibody (PA5-118059) in ICC/IF

Immunofluorescence staining of MFN2 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with MFN2 Polyclonal Antibody (Product # PA5-118059, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

MFN2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MFN2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

MFN2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A549 cells were transfected with MFN2 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-MFN2 Polyclonal Antibody (Product # PA5-118059). {KD}

Product Details

PA5-118059

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:100-1:500

Immunoprecipitation (IP)

1-5 µL/mg of lysate

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived Human MFN2 fragment

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography, Protein A

Storage buffer

PBS, pH 7

Contains

0.03% ProClin 300

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2902662

Target Information

This gene encodes a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI, which are both disorders of the peripheral nervous system. Defects in this gene have also been associated with early-onset stroke. Two transcript variants encoding the same protein have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CPRP 1; hyperplasia suppressor; MFN 2; mitochondrial assembly regulatory factor; Mitofusin-2; Mitofusin2; Transmembrane GTPase MFN2

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Gene Aliases: CMT2A; CMT2A2; CPRP1; HMSN6A; HSG; KIAA0214; MARF; MFN2

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UniProt ID: (Human) O95140

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Entrez Gene ID: (Human) 9927

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Function(s)

GTPase activity protein binding GTP binding ubiquitin protein ligase binding

Process(es)

blastocyst formation protein targeting to mitochondrion apoptotic process response to unfolded protein mitochondrial membrane organization cell cycle arrest blood coagulation mitochondrial fusion macroautophagy protein localization to pre-autophagosomal structure negative regulation of Ras protein signal transduction camera-type eye morphogenesis negative regulation of smooth muscle cell proliferation mitochondrion localization parkin-mediated mitophagy in response to mitochondrial depolarization

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MFN2 Polyclonal Antibody

MFN2 Antibody (PA5-118059) in ICC/IF

Product Details

PA5-118059

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

References (0)

Bioinformatics

Performance Guarantee

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