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OriGene

MCM2 Monoclonal Antibody (OTI1C7), TrueMAB™

View all (72) MCM2 antibodies

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Datasheet
Protocols
Questions & Answers

Cite MCM2 Monoclonal Antibody (OTI1C7), TrueMAB™

  • Antibody Testing Data (2)
MCM2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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MCM2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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FIGURE: 1 / 2

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MCM2 Antibody (CF805621) in IHC (P)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805621) {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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MCM2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
MCM2 Antibody in Western Blot (WB)
MCM2 Monoclonal Antibody (OTI1C7), TrueMAB™

Product Details

CF805621

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:150
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI1C7

Immunogen

Full length human recombinant protein of human MCM2 produced in HEK293T cell.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

MCM2 (Mini chromosome maintenance protein 2) is involved in regulating DNA replication. MCM2 (also called CDCL1, mitotin and BM28), is a human nuclear protein that is crucial in the cell cycle, being involved in the onset of DNA replication and cell division. It is similar to members of the family of early S-phase proteins. MCM2 has been mapped to 3q21. From its localization, CDCL1 became a candidate for an oncogene affected by chromosomal breaks in acute myeloid leukemia (AML).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cell devision cycle-like 1; cyclin-like 1; DNA replication licensing factor MCM2; MGC10606; minichromosome maintenance deficient 2 (mitotin); Minichromosome maintenance protein 2 homolog; Nuclear protein BM28

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Gene Aliases: BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70; KIAA0030; MCM2; MITOTIN

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UniProt ID: (Human) P49736

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Entrez Gene ID: (Human) 4171

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Function(s)
DNA binding DNA helicase activity DNA replication origin binding protein binding ATP binding enzyme binding histone binding metal ion binding
Process(es)
G1/S transition of mitotic cell cycle DNA replication DNA unwinding involved in DNA replication DNA replication initiation nucleosome assembly cell cycle cellular response to interleukin-4
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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