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MAGEB3 Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers

Cite MAGEB3 Polyclonal Antibody

  • Antibody Testing Data (1)
MAGEB3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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MAGEB3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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MAGEB3 Antibody (BS-6823R) in IHC (P)

Paraformaldehyde-fixed, paraffin embedded human melanoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-MAGEB3 Polyclonal Antibody, Unconjugated (bs-6823R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 90 ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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MAGEB3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
MAGEB3 Polyclonal Antibody

Product Details

BS-6823R

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:500
-

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1:500-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide derived from human MAGEB3, amino acids 201-300.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

This gene is a MAGE-B subfamily member of the MAGE gene family. MAGE family member proteins direct the expression of tumor antigens recognized on a human melanoma by autologous cytolytic T lymphocytes. There are two known clusters of MAGE genes on chromosome X. The members of the MAGE-A subfamily are located in the Xq28 region, while the members of the MAGE-B subfamily are clustered in the Xp21 region.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cancer/testis antigen family 3, member 5; MAGE-B3 antigen; melanoma antigen family B, 3; melanoma antigen family B3; Melanoma-associated antigen B3

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Gene Aliases: CT3.5; Mage-b3; Mage-ps1; Mage-rs3; MAGEB3; RGD1562343; Smage3

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UniProt ID: (Human) O15480

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Entrez Gene ID: (Human) 4114, (Mouse) 17147, (Rat) 302746

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Function(s)
molecular_function
Process(es)
biological_process
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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