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  • MAD2 Antibodies

Invitrogen

MAD2 Polyclonal Antibody

View all (25) MAD2 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite MAD2 Polyclonal Antibody

  • Antibody Testing Data (4)
MAD2 Antibody in Immunocytochemistry (ICC/IF)
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MAD2 Antibody in Immunocytochemistry (ICC/IF)
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MAD2 Antibody (PA5-112175) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of MAD2 in A549 cells. Cells were fixed with 4% PFA, permeabilized with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with MAD2 Polyclonal Antibody (Product # PA5-112175) (1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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MAD2 Antibody in Immunocytochemistry (ICC/IF)
MAD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
MAD2 Antibody in Western Blot (WB)
MAD2 Antibody in Immunoprecipitation (IP)

Product Details

PA5-112175

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-

Immunoprecipitation (IP)

1-5 µL/mg of lysate
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived Human MAD2L1 fragment

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography, Protein A

Storage buffer

PBS, pH 7

Contains

0.03% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2866911

Target Information

Tight junction proteins (TJPs) belong to a family of membrane-associated guanylate kinase (MAGUK) homologs that are involved in the organization of epithelial and endothelial intercellular junctions. TJPs bind to the cytoplasmic C termini of junctional transmembrane proteins and link them to the actin cytoskeleton.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: HsMAD2; MAD2 (mitotic arrest deficient, yeast, homolog)-like 1; MAD2-like 1; MAD2-like protein 1; MadL1; Mitotic arrest deficient 2-like protein 1; mitotic arrest deficient, yeast, homolog-like 1; Mitotic spindle assembly checkpoint protein MAD2A

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Gene Aliases: HSMAD2; MAD2; MAD2L1

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UniProt ID: (Human) Q13257

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Entrez Gene ID: (Human) 4085

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Function(s)
protein binding identical protein binding protein homodimerization activity
Process(es)
mitotic sister chromatid segregation sister chromatid cohesion mitotic cell cycle checkpoint mitotic spindle assembly checkpoint anaphase-promoting complex-dependent catabolic process negative regulation of protein catabolic process protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of mitotic cell cycle cell division negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition positive regulation of mitotic cell cycle spindle assembly checkpoint negative regulation of ubiquitin protein ligase activity
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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