Bioss

Lin28A/B Polyclonal Antibody

Name: Lin28A\/B Polyclonal Antibody
Brand: Bioss
SKU: BS-8443R

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)

Lin28A/B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 5

Lin28A/B Antibody (BS-8443R) in IHC (P)

Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Lin28A/B Polyclonal Antibody, Unconjugated (bs-8443R) at 1:400 overnight at 4°C, DAB staining.

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Product Details

BS-8443R

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:400

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500

Immunocytochemistry (ICC/IF)

1:50-1:200

ELISA (ELISA)

1:500-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide derived from human Lin28A.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

LIN-28 is a highly conserved, RNA-binding, cytoplasmic protein. It consists of a cold shock domain and retroviral-type (CCHC) zinc finger motifs that were first identified in Caenorhabditis elegans. LIN-28 controls the timing of events during embryonic development and is readily expressed in embryos, embryonic stem cells and embryonal carcinoma cells. The presence of LIN-28 persists in some adult tissues including cardiac and skeletal muscle. In differentiating myoblasts, LIN-28 increases protein synthesis efficiency and binds to the growth and differentiation factor IGF-II.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: lin-28 homolog B (C. elegans); Lin-28.2; Lin-28A; Lin-28B; Protein lin-28 homolog A; Protein lin-28 homolog B; RNA-binding protein LIN-28; testis expressed gene 17; Testis-expressed protein 17; Zinc finger CCHC domain-containing protein 1; zinc finger, CCHC domain containing 1

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Gene Aliases: 2810403D23Rik; AL024421; CSDD1; CSDD2; D030047M17Rik; ENSMUSG00000070700; Gm10299; LIN-28; Lin-28.2; lin-28A; LIN28; LIN28A; LIN28B; RGD1566408; Tex17; ZCCHC1

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UniProt ID: (Human) Q9H9Z2, (Human) Q6ZN17, (Mouse) Q8K3Y3, (Mouse) Q45KJ6

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Entrez Gene ID: (Human) 79727, (Human) 389421, (Rat) 500562, (Mouse) 83557, (Rat) 689054, (Mouse) 380669

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Function(s)

DNA binding RNA binding mRNA binding protein binding zinc ion binding translation initiation factor binding miRNA binding poly(A) RNA binding molecular_function nucleic acid binding metal ion binding

Process(es)

regulation of transcription, DNA-templated germ cell development biological_process miRNA metabolic process miRNA catabolic process stem cell population maintenance pre-miRNA processing RNA 3'-end processing positive regulation of neuron differentiation negative regulation of glial cell differentiation positive regulation of translation stem cell differentiation regulation of gene silencing by miRNA positive regulation of cell proliferation involved in kidney development gene silencing by RNA somatic stem cell population maintenance

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Lin28A/B Polyclonal Antibody

Lin28A/B Antibody (BS-8443R) in IHC (P)

Product Details

BS-8443R

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Target Information

References (0)

Bioinformatics

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