Invitrogen

LIF Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-21122

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
-

Immunocytochemistry (ICC/IF)

20 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 16 amino acid peptide near the center of human LIF.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11152046

Product Specific Information

A suggested positive control is EL4 cell lysate.

PA5-21122 can be used with blocking peptide PEP-1236.

Target Information

Leukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cholinergic differentiation factor; cholinergic neuronal differentiation factor; D factor; differentiation inhibitory activity; differentiation-inducing factor; Differentiation-stimulating factor; hepatocyte-stimulating factor III; human interleukin in DA cells; Leukemia inhibitory factor; leukemia inhibitory factor (cholinergic differentiation factor); LIF; Melanoma-derived LPL inhibitor; MLPLI; myeloid leukaemia inhibitory factor

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Gene Aliases: CDF; DIA; HILDA; LIF; MLPLI

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UniProt ID: (Human) P15018, (Mouse) P09056

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Entrez Gene ID: (Human) 3976, (Mouse) 16878, (Rat) 60584

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Function(s)
transcription factor activity, RNA polymerase II transcription factor recruiting receptor binding cytokine activity leukemia inhibitory factor receptor binding protein binding growth factor activity
Process(es)
blood vessel remodeling immune response multicellular organism development embryo implantation positive regulation of cell proliferation negative regulation of cell proliferation positive regulation of neuron projection development negative regulation of angiogenesis stem cell population maintenance organ regeneration positive regulation of peptidyl-serine phosphorylation positive regulation of peptidyl-serine phosphorylation of STAT protein tyrosine phosphorylation of Stat3 protein positive regulation of tyrosine phosphorylation of Stat1 protein positive regulation of tyrosine phosphorylation of Stat3 protein positive regulation of MAPK cascade positive regulation of macrophage differentiation negative regulation of meiotic nuclear division positive regulation of transcription from RNA polymerase II promoter decidualization negative regulation of hormone secretion lung alveolus development muscle organ morphogenesis neuron development astrocyte differentiation positive regulation of astrocyte differentiation leukemia inhibitory factor signaling pathway stem cell differentiation positive regulation of peptidyl-tyrosine phosphorylation positive regulation of corticotropin secretion retina development in camera-type eye transdifferentiation lung vasculature development lung lobe morphogenesis trophoblast giant cell differentiation spongiotrophoblast differentiation negative regulation of ERK1 and ERK2 cascade positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis regulation of metanephric nephron tubule epithelial cell differentiation positive regulation of protein localization to nucleus positive regulation of histone H3-K27 acetylation regulation of RNA polymerase II regulatory region sequence-specific DNA binding transcription from RNA polymerase II promoter positive regulation of gene expression lung development regulation of cell differentiation maternal process involved in female pregnancy
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