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  • LEFTY2 Antibodies

Invitrogen

LEFTY2 Polyclonal Antibody

View all (12) LEFTY2 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite LEFTY2 Polyclonal Antibody

  • Antibody Testing Data (3)
LEFTY2 Antibody in Immunocytochemistry (ICC/IF)
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LEFTY2 Antibody in Immunocytochemistry (ICC/IF)
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LEFTY2 Antibody (PA5-103061) in ICC/IF

Immunocytochemistry analysis of LEFTY2 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with LEFTY2 polyclonal antibody (Product # PA5-103061) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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LEFTY2 Antibody in Immunocytochemistry (ICC/IF)
LEFTY2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
LEFTY2 Antibody in Western Blot (WB)
LEFTY2 Polyclonal Antibody

Product Details

PA5-103061

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human LEFTY2(Accession O00292), corresponding to amino acid residues V203-P253.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2852431

Product Specific Information

Antibody detects endogenous levels of total LEFTY2.

Target Information

Left-right determination factor 1 and 2 (Lefty1/2) belong to the TGF-beta superfamily of proteins and are responsible for determination of left-right positioning of visceral organs during embryogenesis. Lefty1 acts as a regulator of Lefty2 and NODAL as the lack of the former resulted in bilateral expression of nodal, lefty2 and Pitx2 (a homeobox gene normally expressed on the left side and blocks the signal for leftness). Lefty2 may play a role in endometrial bleeding.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: endometrial bleeding associated factor; Endometrial bleeding-associated factor; Left-right determination factor 2; Left-right determination factor A; Left-right determination factor B; left-right determination, factor A; MGC46222; Protein lefty-2; Protein lefty-A; Protein lefty-B; TGF-beta-4; Transforming growth factor beta-4

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Gene Aliases: 6030463A22Rik; AV214969; EBAF; Ebaf2; LEFTA; Leftb; LEFTY2; LEFTYA; PSEC0024; TGFB4

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UniProt ID: (Human) O00292, (Mouse) P57785

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Entrez Gene ID: (Human) 7044, (Rat) 289316, (Mouse) 320202

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Function(s)
cytokine activity transforming growth factor beta receptor binding growth factor activity molecular_function nodal binding
Process(es)
platelet degranulation transforming growth factor beta receptor signaling pathway multicellular organism development positive regulation of pathway-restricted SMAD protein phosphorylation cell growth regulation of apoptotic process regulation of MAPK cascade cell development SMAD protein signal transduction negative regulation of transcription from RNA polymerase II promoter heart morphogenesis determination of left/right symmetry biological_process negative regulation of cell proliferation anterior/posterior axis specification response to retinoic acid cell migration involved in gastrulation negative regulation of nodal receptor complex assembly growth
It has to be done as per old AB suggested Products section.
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