Invitrogen

LEF1 Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers
LEF1 Polyclonal Antibody

Product Details

PA5-87521

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of
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Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-132 of human LEF1 (NP_0573531)
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.14 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804221

Product Specific Information

Immunogen sequence: TDEMIPFKDE GDPQKEKIFA EISHPEEEGD LADIKSSLVN ESEIIPASNG HEVARQAQTS QEPYHDKARE HPDDGKHPDG GLYNKGPSYS SYSGYIMMPN MNNDPYMSNG SLS

Positive Samples: Jurkat, Mouse kidney; Cellular Location: Nucleus

Target Information

This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DKFZp586H0919; FLJ46390; HGNC:6551; HMG Binding Domain; LEF-1; lymphoid enhancer binding factor 1 short isoform; Lymphoid enhancer-binding factor 1; OTTHUMP00000219736; OTTHUMP00000219737; T cell-specific transcription factor 1-alpha; TCF1-alpha

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Gene Aliases: 3000002B05; AI451430; LEF-1; LEF1; TCF10; TCF1ALPHA; TCF7L3

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UniProt ID: (Human) Q9UJU2, (Rat) Q9QXN1, (Mouse) P27782

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Entrez Gene ID: (Human) 51176, (Rat) 161452, (Mouse) 16842

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Function(s)
RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding beta-catenin binding transcription factor binding DNA binding, bending estrogen receptor activity estrogen receptor binding enhancer binding histone binding histone deacetylase binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process sequence-specific DNA binding transcription regulatory region DNA binding gamma-catenin binding armadillo repeat domain binding C2H2 zinc finger domain binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter patterning of blood vessels osteoblast differentiation neural crest cell migration somitogenesis kidney development epithelial to mesenchymal transition vasculature development sprouting angiogenesis regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter cell adhesion positive regulation of cell proliferation response to lithium ion positive regulation of gene expression positive regulation of epithelial to mesenchymal transition response to organic cyclic compound Wnt signaling pathway regulation of striated muscle tissue development dentate gyrus development hippocampus development hypothalamus development forebrain radial glial cell differentiation forebrain neuroblast division forebrain neuron differentiation formation of radial glial scaffolds regulation of cell-cell adhesion negative regulation of cell-cell adhesion positive regulation of cell-cell adhesion regulation of Wnt signaling pathway neutrophil differentiation positive regulation of cell growth embryonic limb morphogenesis positive regulation of cell migration BMP signaling pathway positive regulation of granulocyte differentiation mammary gland development organ regeneration negative regulation of interleukin-13 production negative regulation of interleukin-4 production negative regulation of interleukin-5 production T cell receptor V(D)J recombination B cell proliferation odontogenesis of dentin-containing tooth negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of DNA binding steroid hormone mediated signaling pathway tongue development skin development positive regulation by host of viral transcription histone H3 acetylation histone H4 acetylation T-helper 1 cell differentiation negative regulation of striated muscle tissue development negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter alpha-beta T cell differentiation eye pigmentation paraxial mesoderm formation cell development muscle fiber development sensory perception of taste palate development anatomical structure regression canonical Wnt signaling pathway face morphogenesis cell chemotaxis apoptotic process involved in morphogenesis chorio-allantoic fusion trachea gland development cellular response to cytokine stimulus cellular response to interleukin-4 positive regulation of cell proliferation in bone marrow negative regulation of apoptotic process in bone marrow odontoblast differentiation negative regulation of estrogen receptor binding positive regulation of cell cycle process negative regulation of canonical Wnt signaling pathway apoptotic process involved in patterning of blood vessels transcription, DNA-templated regulation of transcription, DNA-templated generation of neurons Wnt signaling pathway, calcium modulating pathway beta-catenin-TCF complex assembly
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