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  • Primary Antibodies ›
  • KDM6A Antibodies

Invitrogen

KDM6A Polyclonal Antibody

Advanced Verification
View all (16) KDM6A antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite KDM6A Polyclonal Antibody

  • Antibody Testing Data (11)
  • Advanced Verification (1)
KDM6A Antibody in Immunocytochemistry (ICC/IF)
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KDM6A Antibody in Immunocytochemistry (ICC/IF)
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KDM6A Antibody (PA5-31828) in ICC/IF

Immunofluorescence analysis of KDM6A was performed using 70% confluent log phase K-562 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with KDM6A Polyclonal Antibody (Product # PA5-31828) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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KDM6A Antibody in Immunocytochemistry (ICC/IF)
KDM6A Antibody in Immunocytochemistry (ICC/IF)
KDM6A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
KDM6A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
KDM6A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
KDM6A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
KDM6A Antibody in Western Blot (WB)
KDM6A Antibody in Western Blot (WB)
KDM6A Antibody in Western Blot (WB)
KDM6A Antibody in Immunoprecipitation (IP)
KDM6A Antibody in ChIP Assay (ChIP)
KDM6A Antibody

Product Details

PA5-31828

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (IHC)

-
- -

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-

Flow Cytometry (Flow)

-
- -

Immunoprecipitation (IP)

1:100-1:500
-

ChIP assay (ChIP)

Assay-Dependent
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human KDM6A. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.78 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2549301

Product Specific Information

Recommended positive controls: SK-N-SH, IMR32, SK-N-AS.

Predicted reactivity: Mouse (93%), Xenopus laevis (82%), Dog (86%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 4lysine (K)-specific demethylase 6A; [histone H3]-trimethyl-L-lysine(27) demethylase 6A; bA386N14.2 (ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX)); Histone demethylase UTX; lysine (K)-specific demethylase 6A; Lysine-specific demethylase 6A; ubiquitously transcribed tetratricopeptide repeat gene, X chromosome; ubiquitously transcribed tetratricopeptide repeat protein X-linked; Ubiquitously transcribed TPR protein on the X chromosome; Ubiquitously transcribed X chromosome tetratricopeptide repeat protein; ubiquitously-transcribed TPR gene on the X chromosome; Ubiquitously-transcribed TPR protein on the X chromosome; Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein

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Gene Aliases: bA386N14.2; KABUK2; KDM6A; UTX

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UniProt ID: (Human) O15550, (Mouse) O70546

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Entrez Gene ID: (Human) 7403, (Mouse) 22289

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding chromatin DNA binding histone demethylase activity identical protein binding metal ion binding dioxygenase activity histone demethylase activity (H3-K27 specific) sulfonate dioxygenase activity chromatin binding protein binding oxidoreductase activity 2,4-dichlorophenoxyacetate alpha-ketoglutarate dioxygenase activity procollagen-proline dioxygenase activity hypophosphite dioxygenase activity DNA-N1-methyladenine dioxygenase activity C-19 gibberellin 2-beta-dioxygenase activity C-20 gibberellin 2-beta-dioxygenase activity
Process(es)
in utero embryonic development neural tube closure heart morphogenesis respiratory system process positive regulation of gene expression somite rostral/caudal axis specification multicellular organism growth mesodermal cell differentiation notochord morphogenesis histone H3-K4 methylation oxidation-reduction process canonical Wnt signaling pathway histone H3-K27 demethylation heart development regulation of gene expression chromatin modification neural tube development embryonic organ development cardiovascular system development
It has to be done as per old AB suggested Products section.
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