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  • KCC2 Antibodies

QED Bioscience

K+/Cl-Cotransporter (KCC2) Monoclonal Antibody (S1-12)

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Datasheet
Protocols
Questions & Answers

Cite K+/Cl-Cotransporter (KCC2) Monoclonal Antibody (S1-12)

  • Antibody Testing Data (3)
K+/Cl-Cotransporter (KCC2) Antibody in Immunocytochemistry (ICC/IF)
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K+/Cl-Cotransporter (KCC2) Antibody in Immunocytochemistry (ICC/IF)
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K+/Cl-Cotransporter (KCC2) Antibody (11564-100UG) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-KCC2 Monoclonal Antibody, Clone S1-12 (11564). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-KCC2 Monoclonal Antibody (11564) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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K+/Cl-Cotransporter (KCC2) Antibody in Immunocytochemistry (ICC/IF)
K+/Cl-Cotransporter (KCC2) Antibody in Immunocytochemistry (ICC/IF)
K+/Cl-Cotransporter (KCC2) Antibody in Western Blot (WB)
K+/Cl-Cotransporter (KCC2) Monoclonal Antibody (S1-12)

Product Details

11564-100UG

Applications
Tested Dilution
Publications

Western Blot (WB)

3 µg/mL
-

Immunohistochemistry (IHC)

1-5 µg/mL
-

Immunocytochemistry (ICC/IF)

1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

S1-12

Immunogen

Fusion protein consisting of aa 932-1043 of rat KCC2 (accession no. NP_599190).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Target Information

Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Electroneutral potassium-chloride cotransporter 2; erythroid K-Cl cotransporter 2; Furosemide-sensitive K-Cl cotransporter; hKCC2; K-Cl cotransporter 2; KCC 2; KCC2a variant 1; KCC2a-S25 variant 1; KCC2b variant 2; mKCC2; Neuronal K-Cl cotransporter; neuronal-specific K-Cl cotransporter; rKCC2; S12A5; solute carrier family 12 (potassium/chloride transporter), member 5; Solute carrier family 12 member 5; solute carrier family 12, member 5

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Gene Aliases: EIEE34; EIG14; hKCC2; KCC2; KIAA1176; mKIAA1176; SLC12A5

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UniProt ID: (Human) Q9H2X9, (Mouse) Q91V14, (Rat) Q63633

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Entrez Gene ID: (Human) 57468, (Mouse) 57138, (Rat) 171373

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Function(s)
chloride transmembrane transporter activity potassium:chloride symporter activity potassium ion symporter activity protein binding ammonium transmembrane transporter activity symporter activity cation:chloride symporter activity protein kinase binding
Process(es)
transport ion transport cellular ion homeostasis chemical synaptic transmission learning cellular chloride ion homeostasis multicellular organism growth thermosensory behavior response to drug dendritic spine development potassium ion transmembrane transport chloride transmembrane transport potassium ion transport chloride transport organic cation transport ammonium transport intracellular pH reduction transmembrane transport ammonium transmembrane transport cation transmembrane transport
It has to be done as per old AB suggested Products section.
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