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  • JMJD1B Antibodies

Invitrogen

JMJD1B Recombinant Rabbit Monoclonal Antibody (1A8)

View all (15) JMJD1B antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite JMJD1B Recombinant Rabbit Monoclonal Antibody (1A8)

  • Antibody Testing Data (5)
JMJD1B Antibody in Immunocytochemistry (ICC/IF)
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JMJD1B Antibody in Immunocytochemistry (ICC/IF)
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JMJD1B Antibody (MA5-44640) in ICC/IF

Immunocytochemistry analysis of KDM3B in HeLa cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 &(8)451;, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Samples were incubated in KDM3B Monoclonal antibody (Product # MA5-44640) using a dilution of 1:100 in 2% negative goat serum overnight at 4 &(8)451; followed by Goat Anti-Rabbit IgG H&L (iFluor™ 488) secondary antibody at a dilution of 1:1,000. PBS instead of the primary antibo... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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JMJD1B Antibody in Immunocytochemistry (ICC/IF)
JMJD1B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
JMJD1B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
JMJD1B Antibody in Western Blot (WB)
JMJD1B Antibody in Flow Cytometry (Flow)
JMJD1B Recombinant Rabbit Monoclonal Antibody (1A8)

Product Details

MA5-44640

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:400-1:2,000
-

Immunocytochemistry (ICC/IF)

1:100
-

Flow Cytometry (Flow)

1:500-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

1A8

Immunogen

Recombinant protein within human KDM3B aa 251-500.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 40% glycerol, 0.1% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931100

Target Information

Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: [histone H3]-dimethyl-L-lysine(9) demethylase 3B; JmjC domain-containing histone demethylation protein 2B; jumonji domain containing 1B; Jumonji domain-containing protein 1B; lysine (K)-specific demethylase 3B; Lysine-specific demethylase 3B; Nuclear protein 5qNCA

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Gene Aliases: 5830462I21Rik; 5qNCA; C5orf7; JHDM2B; JMJD1B; KDM3B; KIAA1082; mKIAA1082; NET22

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UniProt ID: (Human) Q7LBC6, (Mouse) Q6ZPY7

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Entrez Gene ID: (Human) 51780, (Mouse) 277250

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Function(s)
antioxidant activity histone demethylase activity metal ion binding dioxygenase activity sulfonate dioxygenase activity molecular_function oxidoreductase activity 2,4-dichlorophenoxyacetate alpha-ketoglutarate dioxygenase activity procollagen-proline dioxygenase activity hypophosphite dioxygenase activity DNA-N1-methyladenine dioxygenase activity C-19 gibberellin 2-beta-dioxygenase activity C-20 gibberellin 2-beta-dioxygenase activity
Process(es)
transcription, DNA-templated regulation of transcription, DNA-templated biological_process chromatin modification oxidation-reduction process response to cisplatin histone demethylation cellular oxidant detoxification
It has to be done as per old AB suggested Products section.
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