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  • Primary Antibodies ›
  • JMJD1B Antibodies

Invitrogen

JMJD1B Polyclonal Antibody

Advanced Verification
1 Published Figure
1 Reference
View all (15) JMJD1B antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite JMJD1B Polyclonal Antibody

  • Antibody Testing Data (7)
  • Published Figures (1)
  • Advanced Verification (1)
JMJD1B Antibody in Immunocytochemistry (ICC/IF)
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JMJD1B Antibody in Immunocytochemistry (ICC/IF)
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JMJD1B Antibody (PA5-17170) in ICC/IF

Knockdown of JMJD1B was achieved by transfecting IMR-32 cells with JMJD1B specific siRNA (Silencer® select Product # s28657, s224243). Immunofluorescence analysis was performed on untransfected IMR-32 cells (panel a,d), transfected with non-specific scrambled siRNA (panels e, h) and transfected with JMJD1B specific siRNA (panel i, l). Cells were fixed, permeabilized, and labelled with JMJD1B Polyclonal Antibody (Product # PA5-17170, 1:100 dilution) followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Flu... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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JMJD1B Antibody in Immunocytochemistry (ICC/IF)
JMJD1B Antibody in Immunocytochemistry (ICC/IF)
JMJD1B Antibody in Immunocytochemistry (ICC/IF)
JMJD1B Antibody in Immunocytochemistry (ICC/IF)
JMJD1B Antibody in Western Blot (WB)
JMJD1B Antibody in Western Blot (WB)
JMJD1B Antibody in ChIP Assay (ChIP)
JMJD1B Antibody in Western Blot (WB)
JMJD1B Antibody

Product Details

PA5-17170

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100
-

Immunoprecipitation (IP)

1:25
-

ChIP assay (ChIP)

10 µl/10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to the human JMJD1B protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

9.1 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10984495

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: [histone H3]-dimethyl-L-lysine(9) demethylase 3B; JmjC domain-containing histone demethylation protein 2B; jumonji domain containing 1B; Jumonji domain-containing protein 1B; lysine (K)-specific demethylase 3B; Lysine-specific demethylase 3B; Nuclear protein 5qNCA

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Gene Aliases: 5830462I21Rik; 5qNCA; C5orf7; JHDM2B; JMJD1B; KDM3B; KIAA1082; mKIAA1082; NET22

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UniProt ID: (Human) Q7LBC6, (Mouse) Q6ZPY7

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Entrez Gene ID: (Human) 51780, (Mouse) 277250, (Rat) 682469

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Function(s)
antioxidant activity histone demethylase activity metal ion binding dioxygenase activity sulfonate dioxygenase activity molecular_function oxidoreductase activity 2,4-dichlorophenoxyacetate alpha-ketoglutarate dioxygenase activity procollagen-proline dioxygenase activity hypophosphite dioxygenase activity DNA-N1-methyladenine dioxygenase activity C-19 gibberellin 2-beta-dioxygenase activity C-20 gibberellin 2-beta-dioxygenase activity
Process(es)
transcription, DNA-templated regulation of transcription, DNA-templated biological_process chromatin modification oxidation-reduction process response to cisplatin histone demethylation cellular oxidant detoxification
It has to be done as per old AB suggested Products section.
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