Invitrogen

IkB alpha Monoclonal Antibody (F.4.4)

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Datasheet
Protocols
Questions & Answers
IkB alpha Monoclonal Antibody (F.4.4)

Product Details

MA5-15191

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunoprecipitation (IP)

1:50
-
Product Specifications

Species Reactivity

Hamster, Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

F.4.4

Immunogen

Synthetic peptide corresponding to residues near the carboxy-terminus of human IkB alpha
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

871.7 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10978164

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

IkB-alpha is a 40 kDa protein that functions to inhibit NF- kappaB activity. The inhibition occurs via protein-protein interaction between I kappaB proteins and NF- kappaB dimers in the cytosol. The interaction of I kappa B-alpha with NF- kappaB masks the nuclear localization sequence of NF- kappaB, preventing NF- kappaB translocation to the nucleus. A variety of stimuli can activate gene expression by liberating NF- kappaB through the degradation of I kappaB alpha. These stimuli include the proinflammatory cytokines TNF- alpha and IL-1 beta, chemokines, PMA, growth factors, LPS, UV irradiation, viral infection, as well as various chemical and physical stresses. In humans, the gene is located on the q arm of chromosome 14. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase alpha (IKK alpha), previously designated CHUK, interacts with IkB-alpha and specifically phosphorylates IkB-alpha on the sites that trigger its degradation Serines 32 and 36. IKK alpha appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKK alpha is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKK alpha, IKK beta and IKK gamma, and each appear to make essential contributions to IkB phosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: I(Kappa)B(alpha); I-kappa-B-alpha; I-kappaBalpha; IkappaBalpha; IkB-alpha; IKBalpha; Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells alpha; Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells, alpha; Major histocompatibility complex enhancer-binding protein MAD3; NF-kappa-B inhibitor alpha; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, alpha; nuclear factor of kappa light polyp gene enhancer in B-cell 1; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha; RL/IF-1

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Gene Aliases: AI462015; I79_018146; IKBA; MAD-3; MAD3; NFKBI; NFKBIA; RL/IF-1

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UniProt ID: (Human) P25963, (Rat) Q63746, (Mouse) Q9Z1E3

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Entrez Gene ID: (Human) 4792, (Rat) 25493, (Chinese hamster) 100754153, (Mouse) 18035

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Function(s)
protein binding transcription factor binding nuclear localization sequence binding enzyme binding heat shock protein binding ubiquitin protein ligase binding protein complex binding identical protein binding NF-kappaB binding
Process(es)
protein import into nucleus, translocation cytoplasmic sequestering of NF-kappaB regulation of gene expression negative regulation of macrophage derived foam cell differentiation positive regulation of cholesterol efflux negative regulation of lipid storage lipopolysaccharide-mediated signaling pathway negative regulation of NF-kappaB transcription factor activity positive regulation of cellular protein metabolic process response to muramyl dipeptide response to lipopolysaccharide toll-like receptor 4 signaling pathway response to muscle stretch regulation of cell proliferation cytoplasmic sequestering of transcription factor response to exogenous dsRNA negative regulation of myeloid cell differentiation negative regulation of Notch signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter nucleotide-binding oligomerization domain containing 1 signaling pathway nucleotide-binding oligomerization domain containing 2 signaling pathway stimulatory C-type lectin receptor signaling pathway apoptotic process viral process Fc-epsilon receptor signaling pathway regulation of NF-kappaB import into nucleus negative regulation of apoptotic process negative regulation of DNA binding T cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity cellular response to cold cellular response to cytokine stimulus cellular response to organic cyclic compound
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