OriGene

IRS1 Monoclonal Antibody (OTI9E11), TrueMAB™

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Datasheet
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Datasheet
Protocols
Questions & Answers
IRS1 Monoclonal Antibody (OTI9E11), TrueMAB™

Product Details

CF808552

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:150
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI9E11

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-280 of human IRS1 produced in E.coli.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Insulin receptor substrate 1; IRS-1; pp185

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Gene Aliases: G972R; HIRS-1; IRS-1; IRS1; IRS1IRM

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UniProt ID: (Human) P35568, (Rat) P35570, (Mouse) P35569

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Entrez Gene ID: (Human) 3667, (Rat) 25467, (Mouse) 16367

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Function(s)
signal transducer activity transmembrane receptor protein tyrosine kinase adaptor activity protein kinase C binding Ras guanyl-nucleotide exchange factor activity insulin receptor binding insulin-like growth factor receptor binding protein binding 1-phosphatidylinositol-3-kinase activity SH2 domain binding phosphatidylinositol 3-kinase binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein kinase binding protein domain specific binding
Process(es)
MAPK cascade signal transduction positive regulation of cell proliferation insulin receptor signaling pathway positive regulation of glucose metabolic process phosphatidylinositol 3-kinase signaling regulation of phosphatidylinositol 3-kinase signaling positive regulation of fatty acid beta-oxidation response to insulin cellular response to insulin stimulus phosphatidylinositol-3-phosphate biosynthetic process glucose homeostasis response to peptide hormone positive regulation of GTPase activity positive regulation of phosphatidylinositol 3-kinase activity positive regulation of glycogen biosynthetic process positive regulation of glucose import negative regulation of insulin receptor signaling pathway positive regulation of insulin receptor signaling pathway negative regulation of insulin secretion phosphatidylinositol phosphorylation insulin-like growth factor receptor signaling pathway phosphatidylinositol-mediated signaling positive regulation of glucose import in response to insulin stimulus positive regulation of mesenchymal cell proliferation regulation of gene expression lipid catabolic process insulin secretion positive regulation of cell migration mammary gland development response to caffeine protein localization to nucleus positive regulation of phosphorylation protein kinase B signaling protein heterooligomerization positive regulation of glucagon secretion negative regulation of somatostatin secretion
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