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Invitrogen
IL4R (soluble) Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
IL4R (soluble) Antibody (500-P325BT-25UG) in WB
Product Details
500-P325BT-25UG
Species Reactivity
Host/Isotype
Class
Type
Immunogen
Conjugate
Form
Concentration
Purification
Storage buffer
Contains
Storage conditions
Shipping conditions
RRID
Product Specific Information
AA Sequence of recombinant protein: GNMKVLQEPT CVSDYMSIST CEWKMNGPTN CSTELRLLYQ LVFLLSEAHT CIPENNGGAG CVCHLLMDDV VSADNYTLDL WAGQQLLWKG SFKPSEHVKP RAPGNLTVHT NVSDTLLLTW SNPYPPDNYL YNHLTYAVNI WSENDPADFR IYNVTYLEPS LRIAASTLKS GISYRARVRA WAQCYNTTWS EWSPSTKWHN SYREPFEQH.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sIL-4 Receptor alpha. Anti-Human sIL-4 Receptor alpha-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human sIL-4 Receptor alpha by sandwich ELISA (using 100 µL/well), a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sIL-4 Receptor alpha (500-P325) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human sIL-4 Receptor alpha.
Western Blot: To detect Human sIL-4 Receptor alpha by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human sIL-4 Receptor alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P325BT-1MG will be provided as 2 x 500 µg
Target Information
IL4 Receptor alpha (IL4RA) is the alpha chain of the interleukin 4 receptor which binds to both interleukin 4 and interleukin 13 to regulate IgE production, chemokine and mucus production at sites of allergic inflammmation. The IL4 response is involved in promoting Th2 differentiation. The secreted extracellular domain of IL4R alpha, called sIL4R alpha, can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. It has no signaling abilities. Allelic variations in the IL4RA gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Protein Aliases: CD124; il-4 receptor; IL-4 receptor subunit alpha; IL4R nirs variant 1; interleukin-4 receptor alpha chain; Interleukin-4 receptor subunit alpha
Gene Aliases: 582J2.1; CD124; IL-4RA; IL4R; IL4RA
UniProt ID: (Human) P24394
Entrez Gene ID: (Human) 3566
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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