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Invitrogen
IL-4 Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
IL-4 Antibody (500-P94BT-25UG) in WB
Product Details
500-P94BT-25UG
Species Reactivity
Host/Isotype
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Type
Immunogen
Conjugate
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RRID
Product Specific Information
AA Sequence of recombinant protein: MHGCNDSPLR EIINTLNQVT EKGTPCTEMF VPDVLTATRN TTENELICRA SRVLRKFYFP RDVPPCLKNK SGVLGELRKL CRGVSGLNSL RSCTVNESTL TTLKDFLESL KSILRGKYLQ SCTSMS.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat IL-4. Anti-Rat IL-4-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Rat IL-4 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat IL-4 (500-P94) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-4.
Western Blot: To detect Rat IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat IL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P94BT-1MG will be provided as 2 x 500 µg
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Protein Aliases: B-cell growth factor 1; B-cell IGG differentiation factor; B-cell stimulatory factor 1; BSF-1; H-IL-4; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402
Gene Aliases: Il-4; Il4; Il4e12
UniProt ID: (Rat) P20096
Entrez Gene ID: (Rat) 287287
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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