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  • IL-25 Antibodies

Invitrogen

IL-25 Polyclonal Antibody

View all (21) IL-25 antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite IL-25 Polyclonal Antibody

  • Antibody Testing Data (1)
IL-25 Antibody in Western Blot (WB)
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IL-25 Antibody in Western Blot (WB)
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IL-25 Antibody (PA5-79491) in WB

Western blot analysis of IL-25 in Lane 1: recombinant human IL25 protein using 1 ng per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with IL-25 polyclonal antibody (Product # PA5-79491) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat anti-rabbit IgG-HRP secondary antibody at a diluti... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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IL-25 Antibody in Western Blot (WB)
IL-25 Polyclonal Antibody

Product Details

PA5-79491

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

ELISA (ELISA)

1-5 µg/mL (capture)
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived human IL17E recombinant protein (Position: Y33-G177).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2746607

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Target Information

Interleukin 25 (IL-25) is a member of the IL-17 family and is identified as a proinflammatory cytokine which is produced by Th17 effector cells thus enabling the Th17 subset to initiate or maintain the autoimmune process in diseases such as Rheumatoid Arthritis. IL-25 also induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. IL-25 itself is activated through IL-23, IL-5, and TGF-beta.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: il 17e; IL-17E; IL-25; ILN; Interleukin; interleukin 17E; Interleukin-17E; Interleukin-25

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Gene Aliases: IL-17e; IL17E; IL25; RGD1561632; UNQ3120/PRO10272

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UniProt ID: (Human) Q9H293, (Mouse) Q8VHH8

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Entrez Gene ID: (Human) 64806, (Mouse) 140806, (Rat) 501996

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Function(s)
cytokine activity interleukin-17E receptor binding
Process(es)
inflammatory response to antigenic stimulus inflammatory response response to fungus response to nematode eosinophil differentiation positive regulation of transcription from RNA polymerase II promoter cell surface receptor signaling pathway biological_process interleukin-13 production interleukin-5 production
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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