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Invitrogen
IL-25 Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
IL-25 Antibody (500-P89BT-25UG) in WB
Product Details
500-P89BT-25UG
Species Reactivity
Host/Isotype
Class
Type
Immunogen
Conjugate
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Concentration
Purification
Storage buffer
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RRID
Product Specific Information
AA Sequence of recombinant protein: MYSHWPSCCP SKGQDTSEEL LRWSTVPVPP LEPARPNRHP ESCRASEDGP LNSRAISPWR YELDRDLNRL PQDLYHARCL CPHCVSLQTG SHMDPRGNSE LLYHNQTVFY RRPCHGEKGT HKGYCLERRL YRVSLACVCV RPRVMG.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-17E. Anti-Human IL-17E-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human IL-17E by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-17E (500-P89Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-17E.
Western Blot: To detect Human IL-17E by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL.andnbsp; When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-17E is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P89BT-1MG will be provided as 2 x 500 µg
Target Information
Interleukin 25 (IL-25) is a member of the IL-17 family and is identified as a proinflammatory cytokine which is produced by Th17 effector cells thus enabling the Th17 subset to initiate or maintain the autoimmune process in diseases such as Rheumatoid Arthritis. IL-25 also induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. IL-25 itself is activated through IL-23, IL-5, and TGF-beta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Protein Aliases: il 17e; IL-17E; IL-25; ILN; Interleukin; Interleukin-17E; Interleukin-25
Gene Aliases: IL17E; IL25; UNQ3120/PRO10272
UniProt ID: (Human) Q9H293
Entrez Gene ID: (Human) 64806
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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