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  • IL-1 beta Antibodies

Invitrogen

IL-1 beta Polyclonal Antibody, PeproTech®

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View all (119) IL-1 beta antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite IL-1 beta Polyclonal Antibody, PeproTech®

  • Antibody Testing Data (3)
IL-1 beta Antibody in Western Blot (WB)
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IL-1 beta Antibody in Western Blot (WB)
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IL-1 beta Antibody (500-P80-1MG) in WB

Western Blot: To detect Rat IL-1beta by Western Blot analysis IL-1 beta Polyclonal Antibody (Product # 500-P80-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat IL-1beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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IL-1 beta Antibody in Western Blot (WB)
IL-1 beta Antibody in Western Blot (WB)
IL-1 beta Antibody in ELISA (ELISA)
IL-1 beta Polyclonal Antibody, PeproTech®

Product Details

500-P80-1MG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
View 1 publication 1 publication

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL
-

Neutralization (Neu)

-
View 1 publication 1 publication

Miscellaneous PubMed (MISC)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Rat

Published species

Rat

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Rat IL-1beta
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930774

Product Specific Information

AA Sequence of recombinant protein: MVPIRQLHCR LRDEQQKCLV LSDPCELKAL HLNGQNISQQ VVFSMSFVQG ETSNDKIPVA LGLKGLNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKTK VEFESAQFPN WYISTSQAEH RPVFLGNSNG RDIVDFTMEP VSS.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat IL-1beta. Anti-Rat IL-1beta-specific antibody was purified by affinity chromatography employing an immobilized Rat IL-1beta matrix.

Sandwich ELISA: To detect Rat IL-1beta by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat IL-1beta (500-P80Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-1beta.

Western Blot: To detect Rat IL-1beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat IL-1beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Hematopoietin 1 (H1); IFN beta inducing factor; il 1b; IL 1β; IL-1 beta; IL1 B; IL1β; ILN; Interleukin; Interleukin 1 beta precursor; interleukin-1 beta; Interleukin-1b; Interleukin1 beta; LAF; Osteoclast activating factor (OAF); Pro interleukin 1 beta

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Entrez Gene ID: (Rat) 24494

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Function(s)
cytokine activity interleukin-1 receptor binding protein domain specific binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter MAPK cascade activation of MAPK activity fever generation response to hypoxia positive regulation of protein phosphorylation chronic inflammatory response to antigenic stimulus purine nucleobase metabolic process inflammatory response immune response positive regulation of cytosolic calcium ion concentration aging response to nutrient learning or memory memory estrogen metabolic process negative regulation of cell proliferation glycoprotein metabolic process response to heat response to carbohydrate response to ozone response to organonitrogen compound response to gamma radiation positive regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression negative regulation of glucose transport positive regulation of cell death negative regulation of glutamate secretion response to organic cyclic compound cytokine-mediated signaling pathway pentacyclic triterpenoid metabolic process hyaluronan biosynthetic process neutrophil chemotaxis polyketide metabolic process ovulation sequestering of triglyceride positive regulation of fever generation lipopolysaccharide-mediated signaling pathway positive regulation of prostaglandin secretion response to estradiol response to lipopolysaccharide interleukin-1 beta production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interferon-gamma production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of stress-activated MAPK cascade positive regulation of immature T cell proliferation in thymus response to ATP response to vitamin D response to L-ascorbic acid positive regulation of heterotypic cell-cell adhesion social behavior ectopic germ cell programmed cell death positive regulation of myosin light chain kinase activity response to stilbenoid cellular response to drug response to diuretic response to statin wound healing positive regulation of T cell proliferation positive regulation of NF-kappaB import into nucleus response to drug positive regulation of apoptotic process regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of I-kappaB kinase/NF-kappaB signaling response to morphine negative regulation of MAP kinase activity response to peptide hormone protein kinase B signaling positive regulation of JUN kinase activity positive regulation of chemokine biosynthetic process positive regulation of interleukin-2 biosynthetic process positive regulation of interleukin-6 biosynthetic process positive regulation of nitric oxide biosynthetic process response to ethanol negative regulation of neuron differentiation positive regulation of glial cell differentiation positive regulation of angiogenesis negative regulation of lipid metabolic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade negative regulation of insulin receptor signaling pathway positive regulation of astrocyte differentiation positive regulation of phagocytosis negative regulation of neurogenesis regulation of insulin secretion leukocyte migration negative regulation of lipid catabolic process positive regulation of lipid catabolic process positive regulation of membrane protein ectodomain proteolysis positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity response to glucocorticoid positive regulation of cell division positive regulation of calcidiol 1-monooxygenase activity negative regulation of adiponectin secretion positive regulation of ERK1 and ERK2 cascade monocyte aggregation cellular response to lipopolysaccharide cellular response to antibiotic cellular response to mechanical stimulus cellular response to organic substance cellular response to glucose stimulus cellular response to lipid cellular response to fatty acid cellular response to organic cyclic compound cellular response to methotrexate response to dexamethasone positive regulation of monocyte chemotactic protein-1 production positive regulation of neutrophil chemotaxis extrinsic apoptotic signaling pathway in absence of ligand negative regulation of branching morphogenesis of a nerve negative regulation of neural precursor cell proliferation negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
It has to be done as per old AB suggested Products section.
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