Invitrogen

IDO Recombinant Rabbit Monoclonal Antibody (PD00-62)

Name: IDO Recombinant Rabbit Monoclonal Antibody (PD00-62)
Brand: Invitrogen
SKU: MA5-50819

View all (94) IDO antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)

IDO Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

IDO Antibody (MA5-50819) in ICC/IF

Immunocytochemistry analysis of IDO with HeLa cells. Cells were fixed in 4% paraformaldehyde (20 min, room temp), permeabilized with 0.1% Triton X-100 in PBS (5 min, room temp), then blocked with 1% BSA in 10% negative goat serum (1 hr, room temp), incubated with recombinant monoclonal IDO (Product # MA5-50819) at a dilution of 1:100 (in 1% BSA in PBST overnight at 4°C), followed by Goat Anti-Rabbit IgG H&L 488 at 1:1,000 dilution (PBS used in control). Nuclear DNA was labelled in blue with DAPI, Beta tubulin (red) at 1/100 dilution (overn... View More

IDO Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Immunogen image

Immunogen Image IDO Recombinant Rabbit Monoclonal Antibody (PD00-62)

Product Details

MA5-50819

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

PD00-62

Immunogen

Synthetic peptide derived from the C-terminus of human IDO-1 protein aa 350-403/403.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_3092992

Product Specific Information

Positive control: Human endometrial carcinoma tissue, human lymph nodes tissue, human placenta tissue.

Predicted band size: 45 kDa

Subcellular Location: Cytoplasm.

Target Information

IDO1 is an intracellular heme-containing enzyme that catalyzes the oxidative cleavage of the indole ring of several important regulatory molecules like tryptophan, serotonin, and melatonin. By doing this, IDO1 initiates the production of biologically active metabolites, commonly referred to as kynurenines. IDO1 is widely expressed in a variety of human tissues as well as in macrophages and dendritic cells (DCs). In inflammation, interferons (IFNs) act on specific receptors to trigger IDO1 induction. The production of IFN-gamma and induction of IDO1 represent important antimicrobial mechanisms. Degradation and depletion of tryptophan by IDO1 inhibits the growth of viruses, bacteria and parasites. Furthermore, IDO1 plays a complex and crucial role in immunoregulation during infection, pregnancy, autoimmunity, transplantation, and neoplasia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EC 1.13.11.52; IDO-1; indolamine 2,3 dioxygenase; indole 2,3-dioxygenase; indoleamine; Indoleamine 2,3-dioxygenase 1; Indoleamine-2; Indoleamine-pyrrole 2,3-dioxygenase

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Gene Aliases: IDO; IDO-1; IDO1; INDO

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UniProt ID: (Human) P14902

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Entrez Gene ID: (Human) 3620

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Function(s)

tryptophan 2,3-dioxygenase activity electron carrier activity heme binding indoleamine 2,3-dioxygenase activity metal ion binding

Process(es)

immune system process cytokine production involved in inflammatory response positive regulation of T cell tolerance induction positive regulation of chronic inflammatory response positive regulation of type 2 immune response tryptophan catabolic process female pregnancy tryptophan catabolic process to kynurenine response to lipopolysaccharide negative regulation of interleukin-10 production positive regulation of interleukin-12 production multicellular organismal response to stress kynurenic acid biosynthetic process swimming behavior negative regulation of T cell proliferation regulation of activated T cell proliferation oxidation-reduction process negative regulation of T cell apoptotic process positive regulation of T cell apoptotic process

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IDO Recombinant Rabbit Monoclonal Antibody (PD00-62)

IDO Antibody (MA5-50819) in ICC/IF

Product Details

MA5-50819

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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