Invitrogen

ID3 Monoclonal Antibody (2B11)

Name: ID3 Monoclonal Antibody (2B11)
Brand: Invitrogen
SKU: MA1-23242

Advanced Verification

2 Published Figures

3 References

View all (38) ID3 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

ID3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

ID3 Antibody (MA1-23242) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of ID3 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ID3 Monoclonal Antibody (2B11) (Product # MA1-23242) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

ID3 Antibody in Western Blot, ChIP Assay (WB, ChIP)

ID3 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

ID3 Antibody — cANGPTL4 regulates COL1A2 and COL3A1 expression in fibroblasts via a bHLH-dependent signaling mechanism. (a) Immunoblot analysis of indicated proteins from fibroblasts treated with cANGPTL4 (12 µg/mL) in the presence or absence of TGFβ1 (10 ng/mL). β-tubulin, as loading control, was from the same samples. (b,d) Chromatin immunoprecipitation (ChIP) of SBE sites in the human COL1A2 gene promoter region and E-box sites in the human COL1A2 and COL3A1 gene promoter regions using phospho-Smad3 antibodies and scleraxis (ScxA) antibodies respectively. The gene sequences spanning the SBE and E-box sites and a random control sequence were analyzed by PCR from immunoprecipitated chromatin from fibroblasts treated with TGFβ1 in the presence or absence of cANGPTL4 (cANG). Pre-immune serum was used as a control. PCR was performed using chromatin before immunoprecipitation as input. M, 100 bp DNA ladder. (c) A schematic diagram showing the relative positions of Enhancer boxes (E-Boxes) and Smad binding elements (SBE) on the human COL1A2 and COL3A1 proximal promoters. Sequences of E-Boxes and SBE are shown. ChIP PCR primers designed specifically to bind scleraxis along this proximal promoter region are indicated. (e) Relative mRNA expression of ID3 in fibroblasts treated with 12 µg/mL of cANGPTL4 and lysed for mRNA at the indicated time points post-treatment. (f) Relative mRNA expression of ID3 in control (Fctrl) and ID3-knockdown (FδID3) fibroblasts treated for 16 h with 10 ng/mL of TGFβ1 ( {KD}

Immunogen image

Immunogen Image ID3 Monoclonal Antibody (2B11)

Product Details

MA1-23242

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Immunoprecipitation (IP)

1-5 µg/mL

View 1 publication 1 publication

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2B11

Immunogen

The full-length (amino acids 1-119) mouse Id3 gene expressed in E. coli.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.45 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2295760

Product Specific Information

Recommended positive controls: Id3-transfected 293T, Id3 transfected Sol8 muscle cells and recombinant fusion protein.

Target Information

Members of the Id family of basic helix-loop-helix (bHLH) proteins include ID1, ID2, ID3 and ID4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The ID-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since ID1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the ID protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation. It has been shown that expression of each of the ID proteins is strongly dependent on growth factor activation and that reduction of ID mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: bHLHb25; Class B basic helix-loop-helix protein 25; DNA-binding protein inhibitor ID-3; HEIR 1; Helix-loop-helix protein HEIR-1; HLH642; ID 3; ID-like protein inhibitor HLH 1R21; ID-like protein inhibitor HLH 462; Inhibitor of differentiation 3; Inhibitor of DNA binding 3; inhibitor of DNA binding 3, dominant negative helix-loop-helix protein

View more View less

Gene Aliases: 1R21; BHLHB25; HEIR-1; HEIR1; Hlh462; Id-3; ID3; Idb3

View more View less

UniProt ID: (Human) Q02535, (Mouse) P41133

View more View less

Entrez Gene ID: (Human) 3399, (Mouse) 15903

View more View less

Function(s)

transcription factor activity, sequence-specific DNA binding transcription corepressor activity protein binding transcription factor binding protein domain specific binding protein dimerization activity leptomycin B binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter metanephros development regulation of DNA replication transcription, DNA-templated multicellular organism development central nervous system development heart development muscle organ development circadian rhythm response to wounding neuron differentiation epithelial cell differentiation notochord development odontogenesis positive regulation of apoptotic process negative regulation of sequence-specific DNA binding transcription factor activity negative regulation of myoblast differentiation negative regulation of osteoblast differentiation negative regulation of transcription, DNA-templated regulation of cell cycle cellular response to leptomycin B regulation of transcription, DNA-templated rhythmic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

ID3 Monoclonal Antibody (2B11)

ID3 Antibody (MA1-23242) in ICC/IF

Product Details

MA1-23242

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!