Invitrogen

HuR Polyclonal Antibody

Name: HuR Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-19558

Advanced Verification

View all (27) HuR antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
Advanced Verification (1)

HuR Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

HuR Antibody (PA5-19558) in ICC/IF

Immunofluorescence analysis of HUR was performed using 70% confluent log phase HCT 116 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with HUR Rabbit Polyclonal Antibody (Product # PA5-19558) at 1:250 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product... View More

Product Image Gallery

HuR Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. PC-3 cells were transfected with HUR siRNA and a decrease in signal intensity was observed in western blot application using Anti-HUR Polyclonal Antibody (Product # PA5-19558). {KD}

Immunogen image

Immunogen Image HuR Polyclonal Antibody

Product Details

PA5-19558

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

1:300

Immunocytochemistry (ICC/IF)

5 µg/mL

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human HuR / ELAVL1.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10986966

Product Specific Information

This antibody is predicted to react with chicken and cow based on sequence homology.

Store antibody at 4°C for 1-2 weeks. For long-term storage, store at -20°C.

Target Information

The protein encoded by this gene is a member of the ELAVL protein family. This encoded protein contains 3 RNA-binding domains and binds cis-acting AU-rich elements. It destabilizes mRNAs and thereby regulates gene expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R); Elav-like generic protein; ELAV-like protein 1; embryonic lethal, abnormal vision, drosophila, homolog-like 1; Hu antigen R; HU-antigen A; Hu-antigen R; Human antigen R; HuR; MelG

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Gene Aliases: 2410055N02Rik; ELAV1; ELAVL1; Elra; Hua; HUR; MelG; W91709

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UniProt ID: (Human) Q15717, (Mouse) P70372

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Entrez Gene ID: (Human) 1994, (Mouse) 15568

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Function(s)

nucleotide binding RNA binding double-stranded RNA binding mRNA binding mRNA 3'-UTR binding protein binding AU-rich element binding protein kinase binding mRNA 3'-UTR AU-rich region binding protein homodimerization activity poly(A) RNA binding nucleic acid binding

Process(es)

mRNA splicing, via spliceosome multicellular organism development regulation of mRNA stability positive regulation of translation mRNA stabilization negative regulation of gene silencing by miRNA 3'-UTR-mediated mRNA stabilization regulation of stem cell population maintenance regulation of translation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HuR Polyclonal Antibody

HuR Antibody (PA5-19558) in ICC/IF

Product Details

PA5-19558

Western Blot (WB)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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