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  • H3K9me3 Antibodies

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Histone H3K9me3 Polyclonal Antibody

36 References
View all (17) H3K9me3 antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Cite Histone H3K9me3 Polyclonal Antibody

  • Antibody Testing Data (5)
Histone H3K9me3 Antibody in Immunocytochemistry (ICC/IF)
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Histone H3K9me3 Antibody in Immunocytochemistry (ICC/IF)
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FIGURE: 1 / 5

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Histone H3K9me3 Antibody (39161) in ICC/IF

Histone H3 trimethyl Lys9 antibody tested by immunofluorescence. HeLa cells stained at 2 µg/mL with Histone H3 trimethyl Lys9 antibody. Top panel: Histone H3 trimethyl Lys9 antibody. Middle panel: DAPI. Bottom panel: merge. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Histone H3K9me3 Antibody in Immunocytochemistry (ICC/IF)
Histone H3K9me3 Antibody in Western Blot (WB)
Histone H3K9me3 Antibody in ChIP-Sequencing (ChIP-Seq)
Histone H3K9me3 Antibody in ELISA (ELISA)
Histone H3K9me3 Antibody in Dot Blot (DB)
Histone H3K9me3 Polyclonal Antibody

Product Details

39161

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:5,000
View 6 publications 6 publications

Immunocytochemistry (ICC/IF)

1:500-1:1,000
View 3 publications 3 publications

ELISA (ELISA)

Assay-dependent
-

ChIP assay (ChIP)

2-5 µL
View 8 publications 8 publications

ChIP-sequencing (ChIP-Seq)

5 µL
View 8 publications 8 publications

Dot blot (DB)

Assay-dependent
-

Miscellaneous PubMed (MISC)

-
View 11 publications 11 publications

CUT&Tag (CUT&Tag)

1 µL/50 µL reaction
-
Product Specifications

Species Reactivity

Human, Yeast

Published species

Fungi, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This Histone H3 trimethyl Lys9 antibody was raised against a peptide including trimethyl-lysine 9 of histone H3.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum with 30% glycerol

Contains

0.035% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2532132

Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: H3 histone; H3 histone family, member A; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3 K9; H3-clustered histone 13; H3-clustered histone 14; H3-clustered histone 15; H3/b; H3/d; H3/f; H3/j; H3K9; H3Lys9me3; Hht1p; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 1, H3j; histone 2, H3a; histone 2, H3c; Histone 3; Histone H3; Histone H3.1; Histone H3.2; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; Histone H3/o; Histone H3K9me3; methyl Histone 3; methyl Histone H3; Tri-methyl H3; Tri-methyl Histone H3; Tri-Methyl-H3 K9; Tri-Methyl-H3 Lys9; Tri-Methyl-Histone H3 K9; Tri-Methyl-Histone H3 Lys9; Trimethyl H3; Trimethyl Histone H3

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Gene Aliases: BUR5; H3; H3.2; H3/A; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3C1; H3C10; H3C11; H3C12; H3C13; H3C14; H3C15; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3F1K; H3F2; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; HHT1; HHT2; HIST1H3A; HIST1H3B; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; HIST2H3C; HIST2H3D; N2749; SIN2; YBR010W; YBR0201; YNL031C

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UniProt ID: (Baker's yeast) P61830, (Human) P68431, (Human) Q71DI3

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Entrez Gene ID: (Baker's yeast) 852295, (Human) 8350, (Human) 8358, (Human) 8968, (Human) 8357, (Human) 8356, (Human) 333932, (Human) 126961

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Function(s)
DNA binding protein heterodimerization activity protein binding histone binding cadherin binding involved in cell-cell adhesion
Process(es)
chromatin silencing at rDNA nucleosome assembly blood coagulation gene silencing by RNA cellular protein metabolic process negative regulation of gene expression, epigenetic positive regulation of gene expression, epigenetic positive regulation of defense response to virus by host DNA replication-dependent nucleosome assembly telomere organization protein heterotetramerization regulation of gene silencing cell-cell adhesion xenophagy DNA repair chromatin assembly or disassembly cellular response to DNA damage stimulus mitotic spindle assembly checkpoint rRNA transcription sexual sporulation resulting in formation of a cellular spore global genome nucleotide-excision repair
It has to be done as per old AB suggested Products section.
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