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  • Histone H1 Antibodies

NeoBiotechnologies

Histone H1 Recombinant Mouse Monoclonal Antibody (r1415-1)

View all (32) Histone H1 antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite Histone H1 Recombinant Mouse Monoclonal Antibody (r1415-1)

  • Antibody Testing Data (6)
Histone H1 Antibody in Immunocytochemistry (ICC/IF)
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Histone H1 Antibody in Immunocytochemistry (ICC/IF)
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FIGURE: 1 / 6

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Histone H1 Antibody (3005-MSM7-P1) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis of PFA-fixed HeLa cells using Histone H1 Recombinant Monoclonal Antibody (Product # 3005-MSM7-P1) followed by goat anti-Mouse IgG-CF488 (Green). Phalloidin (Red) is used to label cellmembrane.. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Histone H1 Antibody in Immunocytochemistry (ICC/IF)
Histone H1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Histone H1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Histone H1 Antibody in Western Blot (WB)
Histone H1 Antibody in Flow Cytometry (Flow)
Histone H1 Antibody in SDS-PAGE (SDS-PAGE)
Histone H1 Recombinant Mouse Monoclonal Antibody (r1415-1)

Product Details

3005-MSM7-P1

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL
-

Immunocytochemistry (ICC/IF)

1-2 µg/mL
-

Flow Cytometry (Flow)

1-2 µg/10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

r1415-1

Immunogen

Recombinant full-length human Histone H1 protein
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

Target Information

This locus is the ortholog of a mouse protein-coding gene that displays characteristics of a linker histone and is expressed in nuclei of late maturing spermatids. The human locus is expressed; however, the open reading frame has been disrupted by a frameshift so it is unlikely to encode a functional protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: H1 antibody; H1 histone family, member 0; H1 histone family, member 1; H1 histone family, member 2; H1 histone family, member 5; H1 VAR.1; H1 VAR.3; H1 VAR.5; H1(0); H1-0; H1-0 histone; H1.0; H1.0, H1(0), H1-0; H10; H1a; H1b; H1c; H1F3; H1FV; HIST1H1B antibody; HIST1H1D; Histone 1 antibody; histone 1, family 5; histone 1, H1a; histone 1, H1b; histone 1, H1c; histone H1; Histone H1'; Histone H1(0); Histone H1-0; Histone H1.0; Histone H1.1; Histone H1.2; Histone H1.3; Histone H1.5; Histone H1a; Histone H1b; Histone H1c; Histone H1d; Histone H1s-1; Histone H1s-2; Histone H1s-3; MyD196

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Gene Aliases: 0610008C09Rik; D130017D06Rik; H1; H1(0); H1-0; H1-1; H1-2; H1-5; H1.1; H1.2; H1.5; H10; H1A; H1B; H1C; H1F0; H1F1; H1F2; H1F5; H1FV; H1s-1; H1s-3; H1var1; H1var3; HIf5; His1a; HIST1; HIST1H1A; HIST1H1B; HIST1H1C

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UniProt ID: (Human) P07305, (Mouse) P10922, (Rat) P43278, (Human) Q02539, (Human) P16401, (Human) P16403, (Mouse) P43275, (Rat) D4A3K5, (Mouse) P43276, (Rat) D3ZBN0, (Mouse) P15864

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Entrez Gene ID: (Human) 3005, (Mouse) 14958, (Rat) 24437, (Human) 3024, (Human) 3009, (Human) 3006, (Mouse) 80838, (Rat) 291145, (Mouse) 56702, (Rat) 680522, (Mouse) 50708

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Function(s)
protein binding chromatin DNA binding poly(A) RNA binding DNA binding histone deacetylase binding
Process(es)
nucleosome assembly negative regulation of transcription from RNA polymerase II promoter nucleosome positioning muscle organ development chromatin organization positive regulation of cell growth protein stabilization positive regulation of histone H3-K9 methylation establishment of protein localization to chromatin spermatogenesis histone H3-K4 trimethylation histone H3-K27 trimethylation apoptotic DNA fragmentation
It has to be done as per old AB suggested Products section.
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