Invitrogen

HCN1 Monoclonal Antibody (S70), PerCP

Name: HCN1 Monoclonal Antibody (S70), PerCP
Brand: Invitrogen
SKU: MA5-45401

View all (19) HCN1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (6)

HCN1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

HCN1 Antibody (MA5-45401) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with HCN1 monoclonal antibody (Product # MA5-45401) at 1:100 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) HCN1 antibody (D) Composite.

HCN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HCN1 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

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Immunogen image

Immunogen Image HCN1 Monoclonal Antibody (S70), PerCP

Product Details

MA5-45401

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:1,000

Immunocytochemistry (ICC/IF)

1:100

Immunoprecipitation (IP)

Assay-dependent

Antibody Microarray (AM)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

S70

Immunogen

Fusion protein amino acids 778-910 (C terminus) of rat HCN1

View immunogen

Conjugate

PerCP PerCP PerCP

Excitation/Emission Max

482/675 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2931855

Product Specific Information

1 µg/mL of MA5-45401 was sufficient for detection of HCN1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 100kDa. No cross-reactivity against HCN2.

Target Information

HCN1 includes Hyperpolarization-activated cation channels of the HCN gene family, which contribute to spontaneous rhythmic activity in both heart and brain. HCN1 is a member of a family of pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides. HCN1 and HCN2 play an important role for motor learning and neuronal integration by cerebellar Purkinje cells as well as, shaping autonomous activity of single neurons and the periodicity of network oscillations. The HCN1 expression is highly enriched in cerebral cortex, hippocampus, cerebellum, and facial motor nucleus. HCN2 is highly abundant in mamillary bodies, pontine nucleus, ventral cochlear nucleus, and nucleus of the trapezoid body. These variations in regional specificity of HCN channels could generate important differences in neuronal pacemaker activity across brain systems.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: BCNG-1; Brain cyclic nucleotide-gated channel 1; HAC-2; Hyperpolarization-activated cation channel 2; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1

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Gene Aliases: BCNG-1; BCNG1; C630013B14Rik; EIEE24; HAC-2; HAC2; HCN1

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UniProt ID: (Human) O60741, (Mouse) O88704, (Rat) Q9JKB0

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Entrez Gene ID: (Human) 348980, (Mouse) 15165, (Rat) 84390

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Function(s)

intracellular cAMP activated cation channel activity voltage-gated sodium channel activity voltage-gated potassium channel activity potassium channel activity cAMP binding identical protein binding nucleotide binding ion channel activity voltage-gated ion channel activity sodium channel activity protein binding phosphatidylinositol-4,5-bisphosphate binding phosphatidylinositol-3,4,5-trisphosphate binding protein C-terminus binding protein complex binding

Process(es)

regulation of membrane depolarization transport ion transport potassium ion transport sodium ion transport regulation of ion transmembrane transport sodium ion transmembrane transport regulation of membrane potential apical protein localization negative regulation of action potential retinal cone cell development response to calcium ion transmembrane transport cellular response to cAMP potassium ion transmembrane transport regulation of membrane hyperpolarization positive regulation of cation channel activity inflammatory response aging cellular response to interferon-beta maternal behavior response to cisplatin response to L-glutamate regulation of postsynaptic membrane potential

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HCN1 Monoclonal Antibody (S70), PerCP

HCN1 Antibody (MA5-45401) in ICC/IF

Product Details

MA5-45401

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Antibody Microarray (AM)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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