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  • Primary Antibodies ›
  • HCLS1 Antibodies

Invitrogen

HCLS1 Polyclonal Antibody

View all (27) HCLS1 antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite HCLS1 Polyclonal Antibody

  • Antibody Testing Data (2)
HCLS1 Antibody in Western Blot (WB)
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HCLS1 Antibody in Western Blot (WB)
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HCLS1 Antibody (PA5-102594) in WB

Western blot was performed using HCLS1 Polyclonal Antibody (Product # PA5-102594) and a 70 kDa band corresponding to HCLS1 was observed. Whole cell extracts (30 µg lysate) of NK-92 (Lane 1), Jurkat (Lane 2), THP-1 (Lane 3), HeLa (Lane 4), SK-O-V3 (Lane 5) and Hep G2 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX), 12 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the pr... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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HCLS1 Antibody in Western Blot (WB)
HCLS1 Antibody in Western Blot (WB)
HCLS1 Polyclonal Antibody

Product Details

PA5-102594

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human HCLS1(Accession P14317), corresponding to amino acid residues L173-K223.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2851996

Product Specific Information

Antibody detects endogenous levels of total HCLS1.

Target Information

Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Hematopoietic cell-specific LYN substrate 1; Hematopoietic lineage cell-specific protein; LckBP1; p75

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Gene Aliases: AW213261; CTTNL; HCLS1; HS1; lckBP1; p75

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UniProt ID: (Human) P14317, (Mouse) P49710

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Entrez Gene ID: (Human) 3059, (Mouse) 15163, (Rat) 288077

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Function(s)
RNA polymerase II transcription factor binding actin binding protein binding SH3 domain binding protein kinase binding protein complex binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter positive regulation of cell proliferation response to hormone positive regulation of phosphatidylinositol 3-kinase signaling actin filament polymerization erythrocyte differentiation regulation of actin filament polymerization positive regulation of granulocyte differentiation positive regulation of peptidyl-serine phosphorylation positive regulation of tyrosine phosphorylation of STAT protein positive regulation of transcription factor import into nucleus positive regulation of macrophage differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of peptidyl-tyrosine phosphorylation positive regulation of protein kinase B signaling cellular response to cytokine stimulus negative regulation of leukocyte apoptotic process regulation of transcription, DNA-templated intracellular signal transduction positive regulation of actin cytoskeleton reorganization
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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