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  • H3K79me3 Antibodies

Bioss

H3K79me3 Polyclonal Antibody

View all (22) H3K79me3 antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Cite H3K79me3 Polyclonal Antibody

  • Antibody Testing Data (6)
H3K79me3 Antibody in Immunocytochemistry (ICC/IF)
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H3K79me3 Antibody in Immunocytochemistry (ICC/IF)
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H3K79me3 Antibody (BS-53121R) in ICC/IF

Human osteosarcoma (U2OS) cells were stained with the Bioss antibody against H3K79me3 (Cat. No. bs-53121R) and with DAPI. Cells were fixed with 2.5% formaldehyde for 30’ and blocked with PBS/TX-100 containing 1% BSA. Figure A: cells were immunofluorescently labeled with the H3K79me3 antibody (left) diluted 1:300 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa568 or with DAPI (right). Figure B and C: staining of the cells with the H3K79me3 antibody after incubation of the antibody with 2 ng/μl H4K20me3 and H3K79... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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H3K79me3 Antibody in Immunocytochemistry (ICC/IF)
H3K79me3 Antibody in Western Blot (WB)
H3K79me3 Antibody in ChIP Assay (ChIP)
H3K79me3 Antibody in ChIP-Sequencing (ChIP-seq)
H3K79me3 Antibody in ELISA (ELISA)
H3K79me3 Antibody in Dot Blot (DB)
H3K79me3 Polyclonal Antibody

Product Details

BS-53121R

Applications
Tested Dilution
Publications

Western Blot (WB)

1:300-1:5,000
-

Immunocytochemistry (ICC/IF)

Assay-dependent
-

ELISA (ELISA)

1:500-1:1,000
-

ChIP assay (ChIP)

Assay-dependent
-

ChIP-sequencing (ChIP-Seq)

Assay-dependent
-

Dot blot (DB)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Yeast

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

Polyclonal antibody raised in rabbit against Human histone H3 containing the trimethylated lysine 79 (H3K79me3), using a KLH-conjugated synthetic peptide
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.75 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: H3 histone; H3 histone family, member A; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3 K79; H3-clustered histone 13; H3-clustered histone 14; H3-clustered histone 15; H3-clustered histone 2; H3-clustered histone 3; H3-clustered histone 4; H3-clustered histone 6; H3-clustered histone 7; H3/b; H3/d; H3/f; H3/j; H3K79; H3Lys79me3; Hht1p; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 1, H3j; histone 2, H3a; histone 2, H3c; histone 2, H3c2; histone 2, H3ca2; Histone 3; histone cluster 2, H3c2, pseudogene; histone gene complex 1; Histone H3; Histone H3.1; Histone H3.2; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; Histone H3/o; Histone H3K79me3; methyl Histone 3; methyl Histone H3; Tri-methyl H3; Tri-methyl Histone H3; Tri-Methyl-H3 K79; Tri-Methyl-H3 Lys79; Tri-Methyl-Histone H3 K79; Tri-Methyl-Histone H3 Lys79; Trimethyl H3; Trimethyl Histone H3

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Gene Aliases: BUR5; H3; H3-143; H3-291; H3-53; H3-614; H3-B; H3-F; H3.1-221; H3.1-291; H3.1-I; H3.2; H3.2-221; H3.2-614; H3.2-615; H3.2-616; H3/A; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3a; H3b; H3C1; H3C10; H3C11; H3C12; H3C13; H3C14; H3C15; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3f; H3F1K; H3F2; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; H3g; H3h; H3i; HHT1; HHT2; Hist1; HIST1H3A; HIST1H3B; Hist1h3c; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; Hist2h3b; HIST2H3C; Hist2h3c1; Hist2h3c2; Hist2h3c2-ps; Hist2h3ca1; Hist2h3ca2; HIST2H3D; M32461; N2749; SIN2; YBR010W; YBR0201; YNL031C

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UniProt ID: (Baker's yeast) P61830, (Human) P68431, (Human) Q71DI3, (Mouse) P68433, (Mouse) P84228

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Entrez Gene ID: (Baker's yeast) 852295, (Human) 8350, (Human) 8358, (Human) 8968, (Human) 8357, (Human) 8356, (Human) 333932, (Human) 126961, (Mouse) 360198, (Mouse) 319150, (Mouse) 260423, (Mouse) 319152, (Mouse) 97114

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Function(s)
DNA binding protein heterodimerization activity protein binding histone binding cadherin binding involved in cell-cell adhesion
Process(es)
positive regulation of defense response to virus by host nucleosome assembly DNA replication-dependent nucleosome assembly protein heterotetramerization regulation of gene silencing chromatin silencing at rDNA blood coagulation gene silencing by RNA cellular protein metabolic process negative regulation of gene expression, epigenetic positive regulation of gene expression, epigenetic telomere organization cell-cell adhesion xenophagy DNA repair chromatin assembly or disassembly cellular response to DNA damage stimulus mitotic spindle assembly checkpoint rRNA transcription sexual sporulation resulting in formation of a cellular spore global genome nucleotide-excision repair
It has to be done as per old AB suggested Products section.
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