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  • H3K79me3 Antibodies

Invitrogen

H3K79me3 Polyclonal Antibody

Advanced Verification
1 Published Figure
2 References
View all (22) H3K79me3 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite H3K79me3 Polyclonal Antibody

  • Antibody Testing Data (8)
  • Published Figures (1)
  • Advanced Verification (1)
H3K79me3 Antibody in ChIP-Sequencing (ChIP-seq)
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H3K79me3 Antibody in ChIP-Sequencing (ChIP-seq)
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FIGURE: 1 / 10

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H3K79me3 Antibody (49-1020) in ChIP-seq

ChIP was performed as described above using 1 µg of the anti-H3K79me3 antibody (Product # 49-1020). The IP'd DNA was analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the peak distribution along the complete sequence and a 600 kb region of the X-chromosome (figure A and B), in a 2 Mb region from chromosome 11 (figure C), and in a 100 kb region ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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H3K79me3 Antibody in ChIP-Sequencing (ChIP-seq)
H3K79me3 Antibody in Western Blot (WB)
H3K79me3 Antibody in Western Blot (WB)
H3K79me3 Antibody in ChIP Assay (ChIP)
H3K79me3 Antibody in ChIP Assay (ChIP)
H3K79me3 Antibody in ChIP Assay (ChIP)
H3K79me3 Antibody in ELISA (ELISA)
H3K79me3 Antibody in Dot Blot (DB)
H3K79me3 Antibody in Western Blot (WB)
H3K79me3 Antibody

Product Details

49-1020

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 1 publication 1 publication

ELISA (ELISA)

1:500
-

ChIP assay (ChIP)

1-10 µg/1x10^6 cells
View 1 publication 1 publication

ChIP-sequencing (ChIP-Seq)

1 µg per assay
-

Dot blot (DB)

1:100,000
-
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

raised in rabbits against histone H3 containing the trimethylated lysine 79 [K79me3], using a KLH-conjugated synthetic peptide.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.6 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS

Contains

0.05% sodium azide, 0.05% ProClin 300

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533870

Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: H3 histone; H3 histone family, member A; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3 K79; H3-clustered histone 13; H3-clustered histone 14; H3-clustered histone 15; H3-clustered histone 2; H3-clustered histone 3; H3-clustered histone 4; H3-clustered histone 6; H3-clustered histone 7; H3/b; H3/d; H3/f; H3/j; H3K79; H3Lys79me3; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 1, H3j; histone 2, H3a; histone 2, H3c; histone 2, H3c2; histone 2, H3ca2; Histone 3; histone cluster 2, H3c2, pseudogene; histone gene complex 1; Histone H3; Histone H3.1; Histone H3.2; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; Histone H3/o; Histone H3K79me3; methyl Histone 3; methyl Histone H3; Tri-methyl H3; Tri-methyl Histone H3; Tri-Methyl-H3 K79; Tri-Methyl-H3 Lys79; Tri-Methyl-Histone H3 K79; Tri-Methyl-Histone H3 Lys79; Trimethyl H3; Trimethyl Histone H3

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Gene Aliases: H3; H3-143; H3-291; H3-53; H3-614; H3-B; H3-F; H3.1-221; H3.1-291; H3.1-I; H3.2; H3.2-221; H3.2-614; H3.2-615; H3.2-616; H3/A; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3a; H3b; H3C1; H3C10; H3C11; H3C12; H3C13; H3C14; H3C15; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3f; H3F1K; H3F2; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; H3g; H3h; H3i; Hist1; HIST1H3A; HIST1H3B; Hist1h3c; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; Hist2h3b; HIST2H3C; Hist2h3c1; Hist2h3c2; Hist2h3c2-ps; Hist2h3ca1; Hist2h3ca2; HIST2H3D; M32461

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UniProt ID: (Human) P68431, (Human) Q71DI3, (Mouse) P68433, (Mouse) P84228

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Entrez Gene ID: (Human) 8350, (Human) 8358, (Human) 8968, (Human) 8357, (Human) 8356, (Human) 333932, (Human) 126961, (Mouse) 360198, (Mouse) 319150, (Mouse) 260423, (Mouse) 319152, (Mouse) 97114

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Function(s)
DNA binding protein binding histone binding protein heterodimerization activity cadherin binding involved in cell-cell adhesion
Process(es)
chromatin silencing at rDNA nucleosome assembly blood coagulation gene silencing by RNA cellular protein metabolic process negative regulation of gene expression, epigenetic positive regulation of gene expression, epigenetic positive regulation of defense response to virus by host DNA replication-dependent nucleosome assembly protein heterotetramerization regulation of gene silencing telomere organization cell-cell adhesion xenophagy
It has to be done as per old AB suggested Products section.
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