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  • Primary Antibodies ›
  • H3K4me1 Antibodies
Antibody was validated by peptide array and recommended for denaturing applications. SNAP-ChIP™ spike-in validated H3K4me1 antibody is recommended for ChIP.

Invitrogen

H3K4me1 Polyclonal Antibody

Advanced Verification
1 Reference
View all (17) H3K4me1 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite H3K4me1 Polyclonal Antibody

  • Antibody Testing Data (6)
  • Advanced Verification (2)
H3K4me1 Antibody in Immunocytochemistry (ICC/IF)
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H3K4me1 Antibody in Immunocytochemistry (ICC/IF)
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H3K4me1 Antibody (49-1003) in ICC/IF

Immunofluorescence analysis of Methyl-Histone H3 (Lys4) was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Methyl-Histone H3 (Lys4) Polyclonal Antibody (Product # 49-1003) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alex... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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H3K4me1 Antibody in Immunocytochemistry (ICC/IF)
H3K4me1 Antibody in Western Blot (WB)
H3K4me1 Antibody in Western Blot (WB)
H3K4me1 Antibody in ELISA (ELISA)
H3K4me1 Antibody in Peptide array (Array)
H3K4me1 Antibody in Dot Blot (DB)
H3K4me1 Antibody
H3K4me1 Antibody

Product Details

49-1003

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunocytochemistry (ICC/IF)

1:100
-

ELISA (ELISA)

1:100
-

ChIP assay (ChIP)

-
View 1 publication 1 publication

Peptide Array (Array)

1:1,000
-

Dot blot (DB)

1:50,000
-
Product Specifications

Species Reactivity

Human, Mouse, Xenopus, Zebrafish

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Raised against histone H3 containing the monomethylated lysine 4 (H3K4me1), using a KLH-conjugated synthetic peptide.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

<0.1% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533854

Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: H3 histone; H3 histone family, member A; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3 K4; H3-clustered histone 13; H3-clustered histone 14; H3-clustered histone 15; H3-clustered histone 2; H3-clustered histone 3; H3-clustered histone 4; H3-clustered histone 6; H3-clustered histone 7; H3/b; H3/d; H3/f; H3/j; H3K4; H3K4me; H3Lys4me; H3Lys4me1; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 1, H3j; histone 2, H3a; histone 2, H3c; histone 2, H3c2; histone 2, H3ca2; Histone 3; histone cluster 2, H3c2, pseudogene; histone gene complex 1; Histone H3; Histone H3.1; Histone H3.2; Histone H3.3; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; Histone H3/o; Histone H3K4me1; methyl Histone 3; methyl Histone H3; Methyl-H3 K4; Methyl-H3 Lys4; Methyl-Histone H3 K4; Methyl-Histone H3 Lys4; Mono-methyl H3; Mono-methyl Histone H3

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Gene Aliases: H3; H3-143; H3-291; h3-5; H3-53; H3-614; H3-B; H3-F; H3.1-221; H3.1-291; H3.1-I; H3.2; H3.2-221; H3.2-614; H3.2-615; H3.2-616; h3.2a; H3/A; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3a; H3b; H3C1; H3C10; H3C11; H3C12; H3C13; H3C14; H3C15; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3f; H3F1K; H3F2; h3f3a; h3f3b.1; h3f3c; h3f3d; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; H3g; H3h; H3i; h3r; Hist1; HIST1H3A; HIST1H3B; Hist1h3c; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; hist2h3; HIST2H3A; Hist2h3b; HIST2H3C; Hist2h3c1; Hist2h3c2; Hist2h3c2-ps; Hist2h3ca1; Hist2h3ca2; HIST2H3D; M32461; wu:fa25h06; wu:fa96g06; wu:fb07a08; wu:fb36f01; XELAEV_18028537mg; zgc:110292; zgc:174300; zgc:56193; zgc:56418; zgc:64222; zgc:86731

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UniProt ID: (Human) P68431, (Human) Q71DI3, (Mouse) P68433, (Mouse) P84228, (Zebrafish) Q6PI20

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Entrez Gene ID: (Human) 8350, (Human) 8358, (Human) 8968, (Human) 8357, (Human) 8356, (Human) 333932, (Human) 126961, (Mouse) 360198, (Mouse) 319150, (Mouse) 260423, (Mouse) 319152, (Mouse) 97114, (Zebrafish) 550262, (Xenopus) 399088

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Function(s)
DNA binding protein binding histone binding protein heterodimerization activity cadherin binding involved in cell-cell adhesion
Process(es)
positive regulation of defense response to virus by host nucleosome assembly DNA replication-dependent nucleosome assembly protein heterotetramerization regulation of gene silencing chromatin silencing at rDNA blood coagulation gene silencing by RNA cellular protein metabolic process negative regulation of gene expression, epigenetic positive regulation of gene expression, epigenetic telomere organization cell-cell adhesion xenophagy
It has to be done as per old AB suggested Products section.
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