Invitrogen

Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, AP

Name: Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, AP
Brand: Invitrogen
SKU: A18874

View all (79) Goat anti-Rat IgG antibodies

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Datasheet

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Questions & Answers

Antibody Testing Data (1)

Rat IgG (H+L) Cross-Adsorbed Secondary Antibody in Western Blot (WB)

FIGURE: 1 / 1

Rat IgG (H+L) Cross-Adsorbed Secondary Antibody (A18874) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1) and F9 (Lane 2). The blots were probed with Anti-Alpha Tubulin Rat Monoclonal Antibody (Product # MA1-80017, 1 µg/mL) and detected by chemiluminescence alkaline phosphatase (AP) using Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, AP (Product # A18874) at dilutions 1:2,000 (Fig. 1), 1:10,000 (Fig. 2) and 1... View More

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Product Details

A18874

Applications

Tested Dilution

Western Blot (WB)

1:2,000-1:20,000

Immunohistochemistry (IHC)

1:500-1:20,000

ELISA (ELISA)

1:500-1:20,000

Product Specifications

Species Reactivity

Rat

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

AP AP AP

Form

Liquid

Concentration

2.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 1% BSA, 30mM triethanolamine, 5mM MgCl2, 0.1mM ZnCl2

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2535651

Target

IgG

Cross Adsorption

Against human and mouse IgG

Antibody Form

Whole Antibody

Target Information

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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