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  • Goat anti-Rabbit IgG Secondary Antibodies

Invitrogen

Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 660

View all (206) Goat anti-Rabbit IgG antibodies

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Datasheet
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Datasheet
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Questions & Answers

Cite Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 660

  • Antibody Testing Data (2)
Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody in Western Blot (WB)
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Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody in Western Blot (WB)
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Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (A-21073) in WB

Multiplexed fluorescent western blot was performed using Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 660 (Product # A-21073). Whole cell extracts of THP-1 (Lane 1, 2, 3), MCF7 (Lane 4, 5), HeLa (Lane 6) and HEK-293 (Lane 7) were electrophoresed usingNuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP03222BOX). Resolved proteins were transferred onto anitrocellulose membrane (Prod... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody in Western Blot (WB)
Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody in Western Blot (WB)

Product Details

A-21073

Applications
Tested Dilution

Western Blot (WB)

1:10,000

Immunocytochemistry (ICC/IF)

1-10 µg/mL

Flow Cytometry (Flow)

1-10 µg/mL
Product Specifications

Species Reactivity

Rabbit

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

Alexa Fluor™ 660 Alexa Fluor™ 660 Alexa Fluor™ 660
  • Alexa Fluor 350 
  • Alexa Fluor 405 
  • Alexa Fluor 430 
  • Alexa Fluor 488 
  • Alexa Fluor 514 
  • Alexa Fluor 532 
  • Alexa Fluor 546 
  • Alexa Fluor 555 
  • Alexa Fluor 568 
  • Alexa Fluor 594 
  • Alexa Fluor 633 
  • Alexa Fluor 635 
  • Alexa Fluor 647 
  • Alexa Fluor 680 
  • Alexa Fluor 700 
  • Alexa Fluor 750 
  • Alexa Fluor 790 
  • AP
  • APC 
  • Biotin 
  • Biotin-XX
  • Cyanine3
  • Cyanine5 
  • DyLight 350
  • DyLight 405
  • DyLight 488
  • DyLight 550
  • DyLight 594
  • DyLight 633
  • DyLight 650
  • DyLight 680
  • DyLight 755
  • DyLight 800
  • FITC
  • HRP
  • Pacific Blue
  • Pacific Orange
  • PE 
  • PE-Alexa Fluor 610
  • PE-Alexa Fluor 647
  • Rhodamine
  • Rhodamine Red-X
  • Texas Red
  • Texas Red-X
  • TRITC
  • Request custom conjugation

Excitation/Emission Max

663/691 nm View spectra spectra

Form

Liquid

Concentration

2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.5

Contains

5mM sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2535734

Target

IgG

Cross Adsorption

Against human IgG, human serum, mouse IgG, mouse serum and bovine serum

Antibody Form

Whole Antibody

Product Specific Information

Product will be shipped at Room Temperature.

Target Information

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

View product citations for antibody A-21073 on CiteAb

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