Disclaimer

Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

  • Secondary Antibodies ›
  • Goat anti-Rabbit IgG Secondary Antibodies

Invitrogen

Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 750

Advanced Verification
1 Published Figure
View all (206) Goat anti-Rabbit IgG antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Create Citation
Questions & Answers
Datasheet
Create Citation
Questions & Answers

Cite Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 750

  • Antibody Testing Data (3)
  • Published Figures (1)
  • Advanced Verification (1)
Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody in Immunocytochemistry (ICC/IF)
Group 53 Created with Sketch.
Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody in Immunocytochemistry (ICC/IF)
Group 53 Created with Sketch.

FIGURE: 1 / 5

{{ $ctrl.currentElement.advancedVerification.fullName }}
{{ $ctrl.currentElement.advancedVerification.text }}

Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (A-21039) in ICC/IF

Immunofluorescence analysis of Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 750 (Product # A-21039) was performed using MCF 10A (positive model) and T-47D (negative model) cells stained with Vimentin Polyclonal Antibody (Product # PA5-27231). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA f... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
Published figure supplied by benchsci-logo
PMID: {{$ctrl.currentElement.benchSciPubmedId}}
View Product

{{$ctrl.videoDesc}}

{{$ctrl.videoLongDesc}}

Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody in Immunocytochemistry (ICC/IF)
Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody in Western Blot (WB)
Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody in Western Blot (WB)
Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody in Immunocytochemistry (ICC/IF)
Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody

Product Details

A-21039

Applications
Tested Dilution

Western Blot (WB)

0.04-0.2 µg/mL

Immunocytochemistry (ICC/IF)

1-10 µg/mL

Flow Cytometry (Flow)

1-10 µg/mL
Product Specifications

Species Reactivity

Rabbit

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

Alexa Fluor™ 750 Alexa Fluor™ 750 Alexa Fluor™ 750
  • Alexa Fluor 350 
  • Alexa Fluor 405 
  • Alexa Fluor 430 
  • Alexa Fluor 488 
  • Alexa Fluor 514 
  • Alexa Fluor 532 
  • Alexa Fluor 546 
  • Alexa Fluor 555 
  • Alexa Fluor 568 
  • Alexa Fluor 594 
  • Alexa Fluor 633 
  • Alexa Fluor 635 
  • Alexa Fluor 647 
  • Alexa Fluor 660 
  • Alexa Fluor 680 
  • Alexa Fluor 700 
  • Alexa Fluor 790 
  • AP
  • APC 
  • Biotin 
  • Biotin-XX
  • Cyanine3
  • Cyanine5 
  • DyLight 350
  • DyLight 405
  • DyLight 488
  • DyLight 550
  • DyLight 594
  • DyLight 633
  • DyLight 650
  • DyLight 680
  • DyLight 755
  • DyLight 800
  • FITC
  • HRP
  • Pacific Blue
  • Pacific Orange
  • PE 
  • PE-Alexa Fluor 610
  • PE-Alexa Fluor 647
  • Rhodamine
  • Rhodamine Red-X
  • Texas Red
  • Texas Red-X
  • TRITC
  • Request custom conjugation

Excitation/Emission Max

752/776 nm View spectra spectra

Form

Liquid

Concentration

2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.5

Contains

5mM sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2535710

Target

IgG

Cross Adsorption

Against human IgG, human serum, mouse IgG, mouse serum and bovine serum

Antibody Form

Whole Antibody

Product Specific Information

This secondary antibody is designed for fluorescent Western blot detection on various near-infrared fluorescence instruments. This antibody can be used for multi-color and multiplexing detection when using other antibodies conjugated to compatible Alexa Fluor™ dyes and wavelengths. Other applications of this antibody include immunofluorescent and fluorescent imaging applications when using instrumentation with appropriate excitation and detection capabilities.

Product will be shipped at Room Temperature.

Target Information

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

View product citations for antibody A-21039 on CiteAb

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product
It has to be done as per old AB suggested Products section.
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support

Your items have has been added!


Host server : magellan-search-649bfd9c6b-jlsz7:80/100.66.128.142:80.
git-commit: 6001d82975f0049f9efc09ec70b1b2d89229eeb3
git-url: https://github.com/thermofisher/magellan-search
git-branch: release/2.36.2-2025.09.39-1.0