Invitrogen

Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 555

Name: Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor\u2122 555
Brand: Invitrogen
SKU: A-21137

6 Published Figures

View all (19) Goat anti-Mouse IgG2a antibodies

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Datasheet

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Questions & Answers

Antibody Testing Data (3)
Published Figures (6)

Mouse IgG2a Cross-Adsorbed Secondary Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

Mouse IgG2a Cross-Adsorbed Secondary Antibody (A-21137) in ICC/IF

Immunofluorescence analysis of Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 555 (Product # A-21137) was performed using SH-SY5Y (positive model) and HeLa (negative model) cells stained with beta-3 Tubulin Monoclonal Antibody (Product # MA1-118). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for... View More

Mouse IgG2a Cross-Adsorbed Secondary Antibody in Western Blot (WB)

Mouse IgG2a Cross-Adsorbed Secondary Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Representative images of hippocampal sections from an Alzheimer’s disease (AD) patient and a non-AD patient stained with the Tau1 antibody. Each column corresponds to a different boiling time applied to the sample (0, 10, 20, 30 or 40 min). The first and second rows correspond to samples from an AD patient. The third and fourth rows correspond to samples from a non-AD patient. Samples in the first and third rows were boiled in PBS, while those in the second and fourth rows were boiled in citrate buffer. At 0 min of boiling, the wasteosomes from the AD patient were slightly stained with Tau1 (gray arrowheads). The staining with Tau1 in wasteosomes from the AD patient increased at 10 min of boiling (white arrowheads) and disappeared from 20 min of boiling onward (empty arrowheads). Wasteosomes from the non-AD patient were not stained with Tau1 at any boiling time (third and fourth rows, empty arrowheads). The sections of each row were consecutive and the images shown here were obtained from the same hippocampal region. Hoechst staining (blue) was used for nuclear staining. Scale bar: 20 µm. - Image collected and cropped by CiteAb under a CC-BY license from the following publication: Uncovering tau in wasteosomes (corpora amylacea) of Alzheimer's disease patients. Front Aging Neurosci (2023) Image collected and cropped by CiteAb from the following publication (), licensed under a CC BY license.


Representative images of wasteosomes from hippocampal sections of Alzheimer’s disease (AD) patients stained with the anti-p62 (red) and the Tau5 (green) antibodies after 30 min of boiling in PBS (first and second rows) or citrate buffer (third and fourth rows). Staining with the anti-p62 antibody showed the distinctive staining of wasteosomes. Tau staining colocalized with p62 staining in some areas of the wasteosomes (white arrowheads). p62 also colocalized with tau inclusions (white arrows). Hoechst staining (blue) was used for nuclear staining. Single channel (red and green) views are shown below merged images. Scale bar: 20 µm. - Image collected and cropped by CiteAb under a CC-BY license from the following publication: Uncovering tau in wasteosomes (corpora amylacea) of Alzheimer's disease patients. Front Aging Neurosci (2023) Image collected and cropped by CiteAb from the following publication (), licensed under a CC BY license.


(A) Maximum intensity projection image obtained by confocal microscopy of an Alzheimer’s disease (AD) patient hippocampal section stained with the anti-p62 (red) and Tau5 (green) after 30 min of boiling the sample in citrate. Scale bar: 20 µm. (B,C) Frames obtained from Supplementary Videos 1, 2 where the colocalization of p62 and tau can be appreciated from different perspectives. These videos were obtained after a 3D reconstruction from insets B′ and C′ of the Z-stacks obtained by confocal microscopy. - Image collected and cropped by CiteAb under a CC-BY license from the following publication: Uncovering tau in wasteosomes (corpora amylacea) of Alzheimer's disease patients. Front Aging Neurosci (2023) Image collected and cropped by CiteAb from the following publication (), licensed under a CC BY license.

Product Details

A-21137

Applications

Tested Dilution

Western Blot (WB)

1:10,000

Immunohistochemistry (IHC)

1-10 µg/mL

Immunocytochemistry (ICC/IF)

1-10 µg/mL

Flow Cytometry (Flow)

1-10 µg/mL

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Mouse IgG2a

Conjugate

Alexa Fluor™ 555 Alexa Fluor™ 555 Alexa Fluor™ 555

Excitation/Emission Max

553/568 nm View spectra

Form

Liquid

Concentration

2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.5

Contains

5mM sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2535776

Target

IgG2a

Cross Adsorption

Against mouse IgM, mouse IgA, pooled human sera, purified human paraproteins and mouse isotypes IgG1, IgG2b and IgG3 prior to conjugation

Antibody Form

Whole Antibody

Product Specific Information

Product will be shipped at Room Temperature.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 555

Mouse IgG2a Cross-Adsorbed Secondary Antibody (A-21137) in ICC/IF

Product Details

A-21137

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target

Cross Adsorption

Antibody Form

Product Specific Information

Target Information

References (0)

Performance Guarantee

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