Invitrogen

Goat anti-Mouse IgG, IgM (H+L) Secondary Antibody, HRP

Name: Goat anti-Mouse IgG, IgM (H+L) Secondary Antibody, HRP
Brand: Invitrogen
SKU: 31444

View all (12) Goat anti-Mouse IgG/IgM antibodies

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Datasheet

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Questions & Answers

Antibody Testing Data (1)

Mouse IgG, IgM (H+L) Secondary Antibody in Western Blot (WB)

FIGURE: 1 / 1

Mouse IgG, IgM (H+L) Secondary Antibody (31444) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1) and Jurkat (Lane 2). The blots were probed with Anti-SOD1 Mouse Monoclonal Antibody (Product # MA1-105, 0.5µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG, IgM (H+L) Secondary Antibody, HRP conjugate (Product # A24524) at dilutions 1:5,000 (Fig. 1), 1:10,000 (Fig. 2) and 1:20,000 (Fig. 3). A 18 kDa ... View More

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Product Details

31444

Applications

Tested Dilution

Western Blot (WB)

1:10,000-1:200,000

Immunohistochemistry (IHC)

1:500-1:5,000

Immunocytochemistry (ICC/IF)

1:500-1:5,000

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Conjugate

HRP HRP HRP

Form

Lyophilized

Concentration

0.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.6, with 15mg/mL BSA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_228321

Target

IgG/IgM

Antibody Form

Whole Antibody

Product Specific Information

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.

Product # 31444 has been successfully used in Western blot, and ICC applications.

Antibody Specificity: This antibody reacts with heavy chains on mouse IgG and IgM and with the light chains on most mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.

Restoration and Storage: Store product at 4°C until opened. Restore with 2.0 mL distilled water (0.8 mg/mL after restoration). Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pipette. Product may be stored for several weeks at 4°C as undiluted liquid. After dilution, do not use for more than one day.

To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.

Country of Origin: USA

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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