Invitrogen

Goat anti-Mouse IgG (H+L) Secondary Antibody, AP

Name: Goat anti-Mouse IgG (H+L) Secondary Antibody, AP
Brand: Invitrogen
SKU: PA1-28755

View all (240) Goat anti-Mouse IgG antibodies

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Datasheet

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Questions & Answers

Antibody Testing Data (1)

Mouse IgG (H+L) Secondary Antibody in Western Blot (WB)

FIGURE: 1 / 1

Mouse IgG (H+L) Secondary Antibody (PA1-28755) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1) and U-87 MG (Lane 2). The blots were probed with Anti-SOD1 Mouse Monoclonal Antibody (Product # MA1-105, 2 µg/mL) and detected by chemiluminescence of alkaline phosphatase (AP) using Goat anti-Mouse IgG Secondary Antibody, AP conjugate (Product # PA1-28755) at dilutions 1:1,000 (Fig. 1), 1:2,000 (Fig. 2) and 1:5,000 ... View More

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Product Details

PA1-28755

Applications

Tested Dilution

Western Blot (WB)

1:500-1:2500

Immunohistochemistry (IHC)

1:200-1:1,000

ELISA (ELISA)

1:2,000-1:10,000

Dot blot (DB)

Assay-dependent

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Mouse IgG whole molecule.

Conjugate

AP AP AP

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 8, with 1% BSA, 0.001M MgCl2, 0.0001MM ZnCl2, 50% glycerol

Contains

0.1% sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10978120

Target

IgG

Antibody Form

Whole Antibody

Product Specific Information

PA1-28755 detects Mouse IgG from mouse samples.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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