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  • Secondary Antibodies ›
  • Goat anti-Mouse IgG Secondary Antibodies

Invitrogen

Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 750

View all (240) Goat anti-Mouse IgG antibodies

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Datasheet
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Cite Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 750

  • Antibody Testing Data (3)
Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody in Immunocytochemistry (ICC/IF)
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Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody in Immunocytochemistry (ICC/IF)
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Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (A-21037) in ICC/IF

Immunofluorescence analysis of Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 750 (Product # A-21037) was performed using MCF 10A, cells stained with Vimentin Monoclonal Antibody (V9), eBioscience™ (Product # 14-9897-82). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled wit... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody in Immunocytochemistry (ICC/IF)
Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody in Western Blot (WB)
Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody in Western Blot (WB)

Product Details

A-21037

Applications
Tested Dilution

Western Blot (WB)

0.04-0.2 µg/mL

Immunocytochemistry (ICC/IF)

1-10 µg/mL

Flow Cytometry (Flow)

1-10 µg/mL
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

Alexa Fluor™ 750 Alexa Fluor™ 750 Alexa Fluor™ 750
  • Agarose
  • Alexa Fluor 350 
  • Alexa Fluor 405 
  • Alexa Fluor 430 
  • Alexa Fluor 488 
  • Alexa Fluor 514 
  • Alexa Fluor 532 
  • Alexa Fluor 546 
  • Alexa Fluor 555 
  • Alexa Fluor 568 
  • Alexa Fluor 594 
  • Alexa Fluor 633 
  • Alexa Fluor 635 
  • Alexa Fluor 647 
  • Alexa Fluor 660 
  • Alexa Fluor 680 
  • Alexa Fluor 700 
  • Alexa Fluor 790 
  • AP
  • APC 
  • APC-Alexa Fluor 750
  • Biotin 
  • Biotin-XX
  • BODIPY FL
  • Cyanine3
  • Cyanine5 
  • DyLight 350
  • DyLight 405
  • DyLight 488
  • DyLight 550
  • DyLight 594
  • DyLight 633
  • DyLight 650
  • DyLight 680
  • DyLight 755
  • DyLight 800
  • FITC
  • HRP
  • Pacific Blue
  • PE 
  • PE-Alexa Fluor 610
  • Rhodamine
  • Rhodamine Red-X
  • Texas Red-X
  • TRITC
  • Request custom conjugation

Excitation/Emission Max

752/776 nm View spectra spectra

Form

Liquid

Concentration

2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.5

Contains

5mM sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2535708

Target

IgG

Cross Adsorption

Against human IgG and human serum prior to conjugation

Antibody Form

Whole Antibody

Product Specific Information

This secondary antibody is designed for fluorescent Western blot detection on various near-infrared fluorescence instruments. This antibody can be used for multi-color and multiplexing detection when using other antibodies conjugated to compatible Alexa Fluor™ dyes and wavelengths. Other applications of this antibody include immunofluorescent and fluorescent imaging applications when using instrumentation with appropriate excitation and detection capabilities.

Product will be shipped at Room Temperature.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

View product citations for antibody A-21037 on CiteAb

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