Disclaimer

Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

  • Secondary Antibodies ›
  • Goat anti-Human IgG Secondary Antibodies

Invitrogen

Goat anti-Human IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor™ 488

Advanced Verification
View all (164) Goat anti-Human IgG antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Create Citation
Questions & Answers
Datasheet
Create Citation
Questions & Answers

Cite Goat anti-Human IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor™ 488

  • Antibody Testing Data (2)
  • Advanced Verification (2)
Human IgG (H+L) Secondary Antibody in Immunocytochemistry (ICC/IF)
Group 53 Created with Sketch.
Human IgG (H+L) Secondary Antibody in Immunocytochemistry (ICC/IF)
Group 53 Created with Sketch.

FIGURE: 1 / 4

{{ $ctrl.currentElement.advancedVerification.fullName }}
{{ $ctrl.currentElement.advancedVerification.text }}

Human IgG (H+L) Secondary Antibody (A66724) in ICC/IF

Immunofluorescence analysis of Goat-anti-Human IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ 488 (Product # A66724) was performed on fixed and permeabilized T-47D cells blocked with 2% BSA at room temperature and stained with MUC-1/Gatipotuzumab Recombinant Human Monoclonal Antibody (Product # MA5-42157, used at 1:500 dilution) in 0.1% BSA overnight at 4-degree Celsius. This was detec... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
Published figure supplied by benchsci-logo
PMID: {{$ctrl.currentElement.benchSciPubmedId}}
View Product

{{$ctrl.videoDesc}}

{{$ctrl.videoLongDesc}}

Human IgG (H+L) Secondary Antibody in Immunocytochemistry (ICC/IF)
Human IgG (H+L) Secondary Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Human IgG (H+L) Secondary Antibody
Human IgG (H+L) Secondary Antibody

Product Details

A66724

Applications
Tested Dilution

Immunohistochemistry (Paraffin) (IHC (P))

2 µg/mL

Immunocytochemistry (ICC/IF)

1:500-1:1,000
Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Secondary Antibody

Immunogen

Purified Human IgG

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488
  • Alexa Fluor 647 
  • Alexa Fluor Plus 488 
  • Alexa Fluor Plus 647 
  • AP
  • Biotin 
  • FITC
  • HRP
  • PE 
  • Rhodamine
  • Texas Red
  • TRITC
  • Request custom conjugation

Excitation/Emission Max

499/520 nm View spectra spectra

Form

Liquid

Concentration

1.0 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

5mM sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_3251378

Target

IgG

Antibody Form

Whole Antibody

Product Specific Information

This Goat-anti-Human IgG (H+L), Superclonal™ Recombinant Secondary Antibody specifically detects human IgG Heavy and the Light chains with no cross-reactivity to immunoglobulins from other species except moderate cross-reactivity towards Monkey IgG.

Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity, animal origin-free formulation, and lot-to-lot consistency.

Superclonal™ Recombinant antibodies are a defined pool of recombinant monoclonal antibodies, manufactured by selecting and pooling individual clones to provide complementary, specific, and optimal epitope coverage to achieve enhanced performance.

The sensitivity and specificity of each lot is confirmed using ELISA.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Target Information

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

View product citations for antibody A66724 on CiteAb

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product
It has to be done as per old AB suggested Products section.
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support

Your items have has been added!


Host server : magellan-search-649bfd9c6b-jlsz7:80/100.66.128.142:80.
git-commit: 6001d82975f0049f9efc09ec70b1b2d89229eeb3
git-url: https://github.com/thermofisher/magellan-search
git-branch: release/2.36.2-2025.09.39-1.0