Invitrogen

Goat anti-Human IgG (H+L) Secondary Antibody, HRP

Name: Goat anti-Human IgG (H+L) Secondary Antibody, HRP
Brand: Invitrogen
SKU: H10307

View all (164) Goat anti-Human IgG antibodies

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Datasheet

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Questions & Answers

Antibody Testing Data (4)

Human IgG (H+L) Secondary Antibody in Western Blot (WB)

FIGURE: 1 / 4

Human IgG (H+L) Secondary Antibody (H10307) in WB

Western blot (non-reducing) was performed using Goat anti-Human IgG (H+L) Secondary Antibody, HRP (Product # H10307) and a 150 kDa band corresponding to Human IgG was observed in IM-9, ARH-77 and IM-9, ARH-77 conditioned medium&(8)203; (CM) but not in Raji, Jurkat, Molt-4 and Jurkat CM which are known to have low expression. Whole cell lysate (30 µg) of MOLT-4 (Lane 1), Jurkat (Lane 2), Raji (Lane 3), IM-9 ... View More

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Product Details

H10307

Applications

Tested Dilution

Western Blot (WB)

1:2,000

ELISA (ELISA)

Assay-Dependent

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Conjugate

HRP HRP HRP

Form

Liquid

Purification

purified

Storage buffer

PBS with 4mg/mL BSA, 40% glycerol

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2536551

Target

IgG

Antibody Form

Whole Antibody

Target Information

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

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