Invitrogen

F(ab')2-Goat anti-Human IgG (H+L) Secondary Antibody, TRITC

Name: F(ab\')2-Goat anti-Human IgG (H+L) Secondary Antibody, TRITC
Brand: Invitrogen
SKU: A24469

View all (164) Goat anti-Human IgG antibodies

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Datasheet

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Questions & Answers

Antibody Testing Data (2)

Human IgG (H+L) Secondary Antibody in Western Blot (WB)

FIGURE: 1 / 2

Human IgG (H+L) Secondary Antibody (A24469) in WB

Western blot (non-reducing) was performed using F(ab)2-Goat anti-Human IgG (H+L) Secondary Antibody, TRITC (Product # A24469) and a 150 kDa band corresponding to Human IgG was observed in IM-9, ARH-77 and IM-9, ARH-77 conditioned medium&(8)203; (CM) but not in Raji, Jurkat, Molt-4 and Jurkat CM which are known to have low expression. Whole cell lysate (30 µg) of MOLT-4 (Lane 1), Jurkat (Lane 2), Raji (Lane ... View More

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Product Details

A24469

Applications

Tested Dilution

Western Blot (WB)

1:2,000

Immunocytochemistry (ICC/IF)

1:500-1:20,000

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

TRITC TRITC TRITC

Excitation/Emission Max

552/578 nm View spectra

Form

Lyophilized

Concentration

2.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.2, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2535938

Target

IgG

Antibody Form

F(ab')2 Fragment

Product Specific Information

The sensitivity of each lot of antibody is confirmed using ELISA. The specificity of each lot of antibody is confirmed by immunoelectrophoresis (IEP).

Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. 1 year from date of receipt. Prepare working dilution prior to use and then discard. Based on Immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum immunoglobulins.

Target Information

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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