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  • Goat anti-Human IgG Secondary Antibodies

Invitrogen

Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 546

Advanced Verification
View all (164) Goat anti-Human IgG antibodies

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Datasheet
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Questions & Answers

Cite Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 546

  • Antibody Testing Data (4)
  • Advanced Verification (2)
Human IgG (H+L) Cross-Adsorbed Secondary Antibody in Immunocytochemistry (ICC/IF)
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Human IgG (H+L) Cross-Adsorbed Secondary Antibody in Immunocytochemistry (ICC/IF)
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Human IgG (H+L) Cross-Adsorbed Secondary Antibody (A-21089) in ICC/IF

Immunofluorescence analysis of Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 546 was performedusing log phase IM9 cells (IgG producing B-cell line). The cells were fixed with 4% paraformaldehyde for 10 minutes,permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cellswere labeled with Goat anti-Human IgG (H+L) Cross-Adsor... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Human IgG (H+L) Cross-Adsorbed Secondary Antibody in Immunocytochemistry (ICC/IF)
Human IgG (H+L) Cross-Adsorbed Secondary Antibody in Immunocytochemistry (ICC/IF)
Human IgG (H+L) Cross-Adsorbed Secondary Antibody in Western Blot (WB)
Human IgG (H+L) Cross-Adsorbed Secondary Antibody in Western Blot (WB)
Human IgG (H+L) Cross-Adsorbed Secondary Antibody
Human IgG (H+L) Cross-Adsorbed Secondary Antibody

Product Details

A-21089

Applications
Tested Dilution

Western Blot (WB)

1:2,000

Immunohistochemistry (IHC)

1-10 µg/mL

Immunocytochemistry (ICC/IF)

1-10 µg/mL
Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

Alexa Fluor™ 546 Alexa Fluor™ 546 Alexa Fluor™ 546
  • Alexa Fluor 488 
  • Alexa Fluor 555 
  • Alexa Fluor 568 
  • Alexa Fluor 594 
  • Alexa Fluor 633 
  • Alexa Fluor 647 
  • Alexa Fluor Plus 405 
  • Alexa Fluor Plus 488 
  • Alexa Fluor Plus 555 
  • Alexa Fluor Plus 594 
  • Alexa Fluor Plus 647 
  • AP
  • Biotin 
  • FITC
  • HRP
  • Rhodamine
  • TRITC
  • Request custom conjugation

Excitation/Emission Max

561/572 nm View spectra spectra

Form

Liquid

Concentration

2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.5

Contains

5mM sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2535745

Target

IgG

Cross Adsorption

Against mouse, rabbit and bovine serum prior to conjugation

Antibody Form

Whole Antibody

Product Specific Information

Product will be shipped at Room Temperature.

Target Information

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

View product citations for antibody A-21089 on CiteAb

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