Invitrogen

Goat anti-Human IgG Fc Secondary Antibody, FITC

Name: Goat anti-Human IgG Fc Secondary Antibody, FITC
Brand: Invitrogen
SKU: A18818

Advanced Verification

Datasheet

Create Citation

Questions & Answers

Datasheet

Create Citation

Questions & Answers

Antibody Testing Data (3)
Advanced Verification (1)

Human IgG Fc Secondary Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

Human IgG Fc Secondary Antibody (A18818) in ICC/IF

Immunofluorescence analysis of Goat anti-Human IgG Fc Secondary Antibody, FITC (Product # A18818) was performed using anti-Human MUC1 antibody (Product # MA5- 42157) with T-47D (positive) and Caco-2 (negative) cells. The cells were fixed with 4% Paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 2% BSA, then incubated with following primary antibodies at 1:100 dilution at 4 degree celsi... View More

Product Image Gallery

Product Details

A18818

Applications

Tested Dilution

Immunocytochemistry (ICC/IF)

1:250 - 1:20,000

Flow Cytometry (Flow)

1:500-1:20,000

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobin Fc region

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

2.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.8, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2535595

Target

IgG Fc

Antibody Form

Whole Antibody

Product Specific Information

The sensitivity of each lot of antibody is confirmed using ELISA. The specificity of each lot of antibody is confirmed by immunoelectrophoresis (IEP).

Target Information

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more