OriGene

GLI2 Monoclonal Antibody (OTI1F9), TrueMAB™

View all (24) GLI2 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

CF804549

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500
-

Immunohistochemistry (Paraffin) (IHC (P))

1:150
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI1F9

Immunogen

Human recombinant protein fragment corresponding to amino acids 729-1123 of human GLI2 produced in E.coli.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

Gli-2 belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: GLI family zinc finger protein 2; GLI-Kruppel family member GLI2; glioma-associated oncogene family zinc finger 2; oncogene GLI2; Tax helper protein; tax helper protein 1; tax helper protein 2; tax-responsive element-2 holding protein; tax-responsive element-25-bp sequence binding protein; Zinc finger protein GLI2

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Gene Aliases: CJS; GLI2; HPE9; PHS2; THP; THP1; THP2

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UniProt ID: (Human) P10070

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Entrez Gene ID: (Human) 2736

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding zinc ion binding sequence-specific DNA binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter skeletal system development in utero embryonic development kidney development chondrocyte differentiation osteoblast development transcription from RNA polymerase II promoter smoothened signaling pathway pattern specification process axon guidance ventral midline development hindgut morphogenesis heart development cell proliferation regulation of smoothened signaling pathway response to mechanical stimulus anterior/posterior pattern specification proximal/distal pattern formation floor plate formation spinal cord dorsal/ventral patterning ventral spinal cord development cerebellar cortex morphogenesis smoothened signaling pathway involved in ventral spinal cord interneuron specification smoothened signaling pathway involved in spinal cord motor neuron cell fate specification smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation spinal cord ventral commissure morphogenesis pituitary gland development lung development mammary gland development hindbrain development negative regulation of chondrocyte differentiation positive regulation of T cell differentiation in thymus tube development odontogenesis of dentin-containing tooth embryonic digit morphogenesis negative regulation of apoptotic process positive regulation of neuron differentiation positive regulation of DNA replication positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter embryonic digestive tract development developmental growth neuron development branching morphogenesis of an epithelial tube notochord regression prostatic bud formation mammary gland duct morphogenesis smoothened signaling pathway involved in dorsal/ventral neural tube patterning cellular response to organic cyclic compound cochlea morphogenesis
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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