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OriGene

GLI1 Monoclonal Antibody (OTI2D5E2), TrueMAB™

View all (91) GLI1 antibodies

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Datasheet
Protocols
Questions & Answers

Cite GLI1 Monoclonal Antibody (OTI2D5E2), TrueMAB™

  • Antibody Testing Data (2)
GLI1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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GLI1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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GLI1 Antibody (CF803697) in IHC (P)

Immunohistochemical staining of paraffin-embedded human embryonic cerebellum using anti-GLI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803697) {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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GLI1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
GLI1 Antibody in Western Blot (WB)
GLI1 Monoclonal Antibody (OTI2D5E2), TrueMAB™

Product Details

CF803697

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:150
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI2D5E2

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-410 of human GLI1 produced in E.coli.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: GLI-Kruppel family member GLI1; Glioma-associated oncogene; glioma-associated oncogene 1; glioma-associated oncogene homolog 1 (zinc finger protein); Oncogene GLI; Zinc finger protein GLI1

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Gene Aliases: GLI; GLI1

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UniProt ID: (Human) P08151

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Entrez Gene ID: (Human) 2735

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Function(s)
RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding DNA binding chromatin binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding microtubule binding transcription regulatory region DNA binding metal ion binding
Process(es)
osteoblast differentiation transcription, DNA-templated smoothened signaling pathway spermatogenesis ventral midline development positive regulation of cell proliferation regulation of smoothened signaling pathway response to wounding epidermal cell differentiation dorsal/ventral pattern formation proximal/distal pattern formation cerebellar cortex morphogenesis smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation pituitary gland development lung development regulation of osteoblast differentiation positive regulation of DNA replication positive regulation of smoothened signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter digestive tract morphogenesis notochord regression canonical Wnt signaling pathway negative regulation of canonical Wnt signaling pathway liver regeneration regulation of hepatocyte proliferation
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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