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  • Primary Antibodies ›
  • GBF1 Antibodies

Bethyl Laboratories

GBF1 Polyclonal Antibody

View all (12) GBF1 antibodies

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Datasheet
Protocols
Questions & Answers

Cite GBF1 Polyclonal Antibody

  • Antibody Testing Data (2)
GBF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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GBF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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GBF1 Antibody (A301-963A) in IHC (P)

Detection of human GBF1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-GBF1 (Product # A301-963A) (Lot 1) used at a dilution of 1:200 (1 µg/mL). Detection: DAB. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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GBF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
GBF1 Antibody in Western Blot (WB)
GBF1 Polyclonal Antibody

Product Details

A301-963A

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000-1:10,000
-

Immunohistochemistry (IHC)

1:100-1:500
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500
-

Immunoprecipitation (IP)

2-5 µg/mg lysate
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 1809 and 1859 of human golgi-specific brefeldin A resistance factor 1.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.20 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.0 to 8.0, with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 or Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

Target Information

This gene encodes a member of the Sec7 domain family. The encoded protein is a guanine nucleotide exchange factor that regulates the recruitment of proteins to membranes by mediating GDP to GTP exchange. The encoded protein is localized to the Golgi apparatus and plays a role in vesicular trafficking by activating ADP ribosylation factor 1. The encoded protein has also been identified as an important host factor for viral replication. Multiple transcript variants have been observed for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: BFA-resistant GEF 1; Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1

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Gene Aliases: 1700083E03Rik; AI035702; ARF1GEF; AW049900; GBF1; KIAA0248; mKIAA0248

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UniProt ID: (Human) Q92538, (Mouse) Q6DFZ1

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Entrez Gene ID: (Human) 8729, (Mouse) 107338

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Function(s)
guanyl-nucleotide exchange factor activity ARF guanyl-nucleotide exchange factor activity protein binding phosphatidylinositol-3,4,5-trisphosphate binding phosphatidylinositol-3,5-bisphosphate binding
Process(es)
cell activation involved in immune response ER to Golgi vesicle-mediated transport retrograde vesicle-mediated transport, Golgi to ER post-Golgi vesicle-mediated transport Golgi to endosome transport Golgi organization regulation of mitotic cell cycle protein transport viral process neutrophil chemotaxis regulation of ARF protein signal transduction protein localization to Golgi apparatus retrograde transport, endosome to Golgi positive regulation of GTPase activity COPI coating of Golgi vesicle establishment of monopolar cell polarity protein localization to endoplasmic reticulum exit site Golgi disassembly endoplasmic reticulum-Golgi intermediate compartment organization reactive oxygen species biosynthetic process protein localization to endoplasmic reticulum tubular network regulation of protein localization to cell surface regulation of cell adhesion
It has to be done as per old AB suggested Products section.
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