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  • GAPDH Antibodies

Invitrogen

GAPDH Loading Control Monoclonal Antibody (GA1R)

Advanced Verification
496 References
View all (118) GAPDH antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite GAPDH Loading Control Monoclonal Antibody (GA1R)

  • Antibody Testing Data (16)
  • Advanced Verification (1)
GAPDH Loading Control Antibody in Immunocytochemistry (ICC/IF)
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GAPDH Loading Control Antibody in Immunocytochemistry (ICC/IF)
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GAPDH Loading Control Antibody (MA5-15738) in ICC/IF

Immunofluorescent analysis of GAPDH (green) in HeLa. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 5% normal goat serum (Product # 31873) for 15 minutes at room temperature. Cells were probed with a GAPDH monoclonal antibody (Product # MA5-15738) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and in... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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GAPDH Loading Control Antibody in Immunocytochemistry (ICC/IF)
GAPDH Loading Control Antibody in Immunohistochemistry (Paraffin) (IHC (P))
GAPDH Loading Control Antibody in Western Blot (WB)
GAPDH Loading Control Antibody in Flow Cytometry (Flow)
GAPDH Loading Control Antibody in Immunocytochemistry (ICC/IF)
GAPDH Loading Control Antibody in Immunohistochemistry (Paraffin) (IHC (P))
GAPDH Loading Control Antibody in Flow Cytometry (Flow)
GAPDH Loading Control Antibody in Immunocytochemistry (ICC/IF)
GAPDH Loading Control Antibody in Immunocytochemistry (ICC/IF)
GAPDH Loading Control Antibody in Immunohistochemistry (Paraffin) (IHC (P))
GAPDH Loading Control Antibody in Western Blot (WB)
GAPDH Loading Control Antibody in Western Blot (WB)
GAPDH Loading Control Antibody in Western Blot (WB)
GAPDH Loading Control Antibody in Western Blot (WB)
GAPDH Loading Control Antibody in Western Blot (WB)
GAPDH Loading Control Antibody in Flow Cytometry (Flow)
GAPDH Loading Control Antibody

Product Details

MA5-15738

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:10,000
View 407 publications 407 publications

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500
View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:20-1:200
View 64 publications 64 publications

Flow Cytometry (Flow)

2 µg/test
View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent
-

Immunoprecipitation (IP)

-
View 2 publications 2 publications

Functional Assay (Functional)

-
View 1 publication 1 publication

in situ PLA (PLA)

-
View 2 publications 2 publications

In vitro Assay (IV)

-
View 1 publication 1 publication

Miscellaneous PubMed (MISC)

-
View 15 publications 15 publications
Product Specifications

Species Reactivity

Bacteria, Dog, Chicken, Hamster, Human, Insect, Mouse, Rabbit, Rat, Yeast

Published species

Bacteria, Chicken, Dog, Fruit fly, Horse, Human, Mouse, Non-human primate, Pig, Protozoa, Rabbit, Rat, Rhesus monkey, Rodent, Yeast, Zebrafish

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

GA1R

Immunogen

Recombinant GAPDH

Conjugate

Unconjugated Unconjugated Unconjugated
  • Alexa Fluor 488 
  • Alexa Fluor 555 
  • Alexa Fluor 647 
  • Biotin 
  • DyLight 488
  • DyLight 550
  • DyLight 650
  • DyLight 680
  • DyLight 800 4X PEG
  • HRP
  • Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10977387

Product Specific Information

This antibody detects GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, hamster, and chicken samples.

MA5-15738 has been successfully used in Western blot, ICC, IF, IHC (P), FACS and ELISA.

MA5-15738 may be stored at 4°C short term. Add 0.05% sodium azide if desired. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Target Information

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 38 kDa BFA-dependent ADP-ribosylation substrate; aging-associated gene 9 protein; BARS-38; EC 1.2.1.12; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; GAPDH; GAPDH-2; Glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase type 2; glyceraldehyde-3-phosphate dehydrogenase, spermatogenic; Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; HGNC:4141; MGC88685; Peptidyl-cysteine S-nitrosylase GAPDH; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase

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Gene Aliases: BARS-38; CDABP0047; G3PD; GAPD; Gapd-s; GAPD2; GAPDH; GAPDH-2; GAPDH2; GAPDHS; GAPDS; HEL-S-162eP; HEL-S-278; HSD-35; HSD35; I79_001391; KNC-NDS6; OK/SW-cl.12

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UniProt ID: (Rabbit) P46406, (Human) P04406, (Chicken) P00356, (Human) O14556, (Mouse) P16858, (Chinese hamster) P17244, (Rat) P04797, (Rat) Q9ESV6, (Mouse) Q64467

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Entrez Gene ID: (Rabbit) 100009074, (Human) 2597, (Chicken) 374193, (Dog) 403755, (Human) 26330, (Dog) 476483, (Rabbit) 100352213, (Mouse) 14433, (Chinese hamster) 100736557, (Rat) 24383, (Chinese hamster) 100767862, (Rat) 66020, (Mouse) 14447

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Function(s)
glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity protein binding microtubule binding peptidyl-cysteine S-nitrosylase activity identical protein binding NADP binding NAD binding molecular_function oxidoreductase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
Process(es)
gluconeogenesis spermatid development sperm motility positive regulation of glycolytic process canonical glycolysis microtubule cytoskeleton organization regulation of macroautophagy negative regulation of translation peptidyl-cysteine S-trans-nitrosylation protein stabilization neuron apoptotic process cellular response to interferon-gamma carbohydrate metabolic process glycolytic process apoptotic process multicellular organism development oxidation-reduction process response to ammonium ion nuclear membrane fusion negative regulation of apoptotic process small molecule metabolic process glucose metabolic process regulation of translation
It has to be done as per old AB suggested Products section.
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